Fatty acid production in cell-free systems
The present invention is directed to methods, compositions, and systems for the production of fatty acids (FAs). Specifically, the disclosed methods, compositions, and systems incorporate algal cell lysates, providing a cell-free environment that generates high levels of FAs for extended periods of time with the addition of liquid media and a carbon source.
1. A method of generating fatty acids in a system, the method comprising:
providing an organic carbon source to an algal cell lysate, wherein the organic carbon source is selected from citrate, acetate, glucose, fructose, galactose, sucrose, maltose, and lactose, and wherein the algal cell lysate is derived from one or more Chlorophyta algae cells from at least one of the following genera: Botryococcus, Chlamydomonas, Chlorella, Chlorococcum, Dunaliella, Haematococcus, Monoraphidium, Muriellopsis, Neochloris, Oocystis, Pseudokirchneriella, Scenedesmus , and Tetraselmis,
providing a liquid environment to the algal cell lysate sufficient to permit mixing of the carbon source with the lysate, and
maintaining the system for sufficient time to permit elongation of fatty acids.
2. The method of claim 1 , wherein the one or more algal cells in the genus Botryococcus is Botryococcus braunii.
3. The method of claim 1 , wherein the lysate is obtained by disrupting the cell membrane of one or more intact algae cells.
4. The method of claim 3 , wherein the algae cell membrane is disrupted mechanically, sonically, or chemically.
5. The method of claim 4 , wherein the algal cell lysate is substantially free of intact cell membrane.
6. The method of claim 1 , wherein the organic carbon source is provided continuously or intermittently over time.
7. The method of claim 6 , wherein the carbon source is provided over a period of at least 1 to 110 days.
8. The method of claim 1 , wherein the liquid environment comprises sufficient nutrients to permit elongation of fatty acids chains.
9. The method of claim 1 , wherein maintaining the system comprises agitating the liquid environment.
10. The method of claim 8 , wherein the liquid environment is agitated continuously or intermittently over time.
11. The method of claim 1 , further comprising isolating the fatty acids.