Plant resistant to
The present invention provides an improved Helminthosporium turcicum -resistant plant, in particular a maize plant which comprises a polynucleotide with one or more resistance-conferring genes, for example on a truncated chromosome fragment from the accession Pepitilla, as well as a cell, a tissue, a part, grain and seeds thereof, an isolated polynucleotide which comprises one or more resistance-conferring genes against Helminthosporium turcicum , a vector, a transgenic plant cell and a transgenic plant containing this polynucleotide. Furthermore, the invention encompasses suitable markers and their use in introducing resistance or the transgene into a plant, as well as the identification of improved maize plants which comprise a truncated chromosome fragment.
1. A maize plant into the genome of which a chromosome fragment from a donor Pepitilla has been integrated,
wherein the chromosome fragment comprises an interval of the donor which exhibits at least the donor allele of the marker MA0008 and comprises a polynucleotide which confers resistance to Helminthosporium turcicum in the maize plant; and
wherein the chromosome fragment:
a) does not comprise an interval of the donor defined by the marker SYN14136 and the marker SYN24931;
b) does not comprise an interval of the donor defined by the marker PZE-108093748 and the marker MA0005; and
c) does not comprise an interval of the donor defined by the marker MA0006 and the marker PZE-108107671,
wherein the polynucleotide which confers resistance to Helminthosporium turcicum comprises:
(a) a nucleotide sequence with the cDNA of SEQ ID NO: 1;
(b) a nucleotide sequence which is at least 99% identical to the nucleotide sequence with the cDNA of SEQ ID NO: 1; or
(c) a nucleotide sequence coding for a polypeptide with the amino acid sequence of SEQ ID NO: 2; and wherein the donor allele of the marker MA0008 at position 152045141 as projected to B73 reference genome AGPv02 is a thymine (T).
2. The maize plant as claimed in claim 1 , wherein a flowering time for the maize plant and/or a silage yield of the maize plant corresponds to that of a comparative maize plant into the genome of which the chromosome fragment from the donor Pepitilla has not been integrated.
3. A cell, a tissue, or a part of the maize plant as claimed in claim 1 , the cell, tissue, or part comprising the chromosome fragment comprising the interval of the donor which exhibits at least the donor allele of the marker MA0008 and comprising the polynucleotide which confers resistance to Helminthosporium turcicum in the maize plant.
4. A grain or a seed from the maize plant as claimed in claim 1 , the grain or seed comprising the chromosome fragment comprising the interval of the donor which exhibits at least the donor allele of the marker MA0008 and comprising the polynucleotide which confers resistance to Helminthosporium turcicum in the maize plant.
5. The maize plant as claimed in claim 1 , wherein the chromosome fragment does not comprise an interval of the donor defined by and including the marker SYN14136 and the marker SYN24931, and wherein the markers SYN14136 and SYN24931 are part of the interval.
6. The maize plant as claimed in claim 1 , wherein the chromosome fragment does not comprise an interval of the donor defined by and including the marker PZE-108093748 and the marker MA0005, and wherein the markers PZE-108093748 and MA0005 are part of the interval.
7. The maize plant as claimed in claim 1 , wherein the chromosome fragment does not comprise an interval of the donor defined by and including the marker MA0006 and the marker PZE-108107671, and wherein the markers MA0006 and PZE-108107671 are part of the interval.