Transcriptional control in alicyclobacillus acidocaldarius and associated genes, proteins, and methods
Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.
1. An expression vector comprising a nucleic acid encoding a polypeptide having at least 95% sequence identity to SEQ ID NO: 1497 and wherein the polypeptide is a central glycolytic genes regulator.
2. The expression vector of claim 1 , wherein the polypeptide is a central glycolytic genes regulator at a temperature at or above 50 degrees Celsius.
3. The expression vector isolated of claim 1 , wherein the polypeptide has 100% identity to SEQ ID NO: 1497.
4. The expression vector isolated of claim 1 , wherein the nucleic acid has at least 95% identity to SEQ ID NO: 1498.
5. The expression vector isolated of claim 1 , wherein the nucleic acid has 100% identity to SEQ ID NO: 1498.
6. A cell comprising the expression vector of claim 1 .
7. A method of modulating transcription or transcriptional control at temperatures at or above 25 degrees Celsius, the method comprising providing to a transcriptional system the expression vector of claim 1 .
8. The method according to claim 7 , wherein providing to a transcriptional system the expression vector of claim 1 occurs at a temperature at or above 50 degrees Celsius.