Fibronectin type III domain based scaffold compositions, methods and uses
A protein scaffold based on a consensus sequence of fibronectin type III (FN3) proteins, such as the tenth FN3 repeat from human fibronectin (human Tenascin), including isolated nucleic acids that encode a protein scaffold, vectors, host cells, and methods of making and using thereof have applications in diagnostic and/or therapeutic compositions, methods and devices. In particular, protein scaffold molecules binding to IgG have been identified as useful for diagnostic and/or therapeutic applications.
1. An isolated nucleic acid molecule comprising a nucleotide sequence encoding a protein scaffold comprising the amino acid sequence of SEQ ID NO:16.
2. An isolated nucleic acid vector comprising the isolated nucleic acid molecule of claim 1 .
3. A prokaryotic or eukaryotic host cell comprising the isolated nucleic acid molecule of claim 1 .
4. The host cell of claim 3 , wherein said host cell is at least one selected from E. coli BL21Star(DE3), other E. coli cell, yeast, COS-1, COS-7, HEK293, BHK21, CHO, BSC-1, Hep G2, 653, SP2/0, 293, HeLa, myeloma, or lymphoma cells, or any derivative, immortalized or transformed cell thereof.
5. The isolated nucleic acid molecule of claim 1 , comprising the nucleotide sequence set forth in SEQ ID NO:17.
6. An isolated nucleic acid vector comprising the isolated nucleic acid molecule of claim 5 .
7. A prokaryotic or eukaryotic host cell comprising the isolated nucleic acid molecule of claim 5 .
8. The host cell of claim 7 , wherein said host cell is at least one selected from E. coli BL21Star(DE3), other E. coli cell, yeast, COS-1, COS-7, HEK293, BHK21, CHO, BSC-1, Hep G2, 653, SP2/0, 293, HeLa, myeloma, or lymphoma cells, or any derivative, immortalized or transformed cell thereof.
9. An isolated nucleic acid molecule comprising a nucleotide sequence encoding a protein scaffold based on a fibronectin type III (FN3) domain comprising:
a backbone portion having an amino acid sequence identical to SEQ ID NO:16 at residues 1-21, 29-74, and 82-89;
a B:C loop portion between residues 21 and 29 of SEQ ID NO:16 having at least 75% identity to residues 22-28 of the amino acid sequence of SEQ ID NO:16; and
an F:G loop portion between residues 74 and 82 of SEQ ID NO:16 having at least 75% identity to residues 75-81 of the amino acid sequence of SEQ ID NO:16.
10. An isolated nucleic acid vector comprising the isolated nucleic acid molecule of claim 9 .
11. A prokaryotic or eukaryotic host cell comprising the isolated nucleic acid molecule of claim 9 .
12. The host cell of claim 11 , wherein said host cell is at least one selected from E. coli BL21Star(DE3), other E. coli cell, yeast, COS-1, COS-7, HEK293, BHK21, CHO, BSC-1, Hep G2, 653, SP2/0, 293, HeLa, myeloma, or lymphoma cells, or any derivative, immortalized or transformed cell thereof.