Microdissection viewing system
The present disclosure provides devices, systems and methods for the acquisition and display of captured and/or saved slide images of a sample. In particular embodiments, the images are displayed on a portable computer attached to a removable device configured to hold a slide of the sample. The device is aligned with the portable computer so that the displayed sample image is overlaid with the sample being held by the device. In exemplary embodiments, the displayed image is used as a template for dissection of the sample being held by the device.
1. A microdissection method comprising:
a) providing to an imaging device comprising a microscope a first sample of a specimen comprising an area of interest for microdissection;
b) acquiring image data using the imaging device from a scan of the first sample, wherein the scan of the first sample is obtained at a high resolution using a digital acquisition device;
c) digitally processing the image data at the high resolution to obtain a low resolution copy of the image data, or acquiring low resolution image data of the first sample from the scan of the first sample at the low resolution, and displaying an image of the first sample from the low resolution copy or the acquired low resolution image data on a monitor of a viewing terminal;
d) storing the image data from the scan in a database;
e) retrieving on a portable computer stored image data from the area of interest of the first sample, wherein the retrieved image data is presented on a screen of the portable computer as a display image;
f) placing a second sample of the specimen comprising the area of interest for microdissection on a sleeve positioned over the screen of the portable computer;
g) overlaying the area of interest for microdissection of the second sample with the display image of the first sample; and
h) dissecting the area of interest of the second sample using the display image of the first area of interest of the first sample as a template.
2. The method of claim 1 , wherein the first sample of the specimen is stained with a dye and the second sample of the specimen is not stained with a dye.
3. The method of claim 1 , wherein acquiring image data from the scan of the first sample comprises advancing a field of view of an objective lens of the imaging device stepwise across the first sample while acquiring successive images of each field of view.
4. The method of claim 1 , comprising transferring the image data from the scan to a viewing terminal for display to a user on a monitor, wherein the user views the display of the image data and defines the area of interest for microdissection with an annotation.
5. The method of claim 4 , wherein the annotation is stored with the image data on the database, and wherein the stored annotation is retrieved with the image data by the portable computer.
6. The method of claim 1 , comprising selecting of an area of the image of the first sample displayed on the monitor of the viewing terminal, wherein selection of said area prompts transfer of corresponding high resolution image data of the area to the viewing terminal for display on the monitor.
7. The method of claim 1 , wherein a user views the image of the first sample displayed on the monitor of the viewing terminal and annotates the area of interest for microdissection on the low resolution image copy or the acquired low resolution image, the high resolution image, or both; and wherein the annotation is stored on the database and retrieved on the portable computer.
8. The method of claim 1 , wherein the digital acquisition device comprises a camera, a CCD, a linear array, or combinations thereof.