Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
View Patent ↗The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.
1. A preparatory apparatus comprising:
one or more racks, each rack comprising a plurality of lanes, wherein each lane comprises a slot shaped and sized to receive a holder, and an aperture shaped and sized to receive a sample tube;
a plurality of sample tubes and a plurality of holders, wherein each sample tube is removably mounted in one of the one or more racks, wherein each holder is removably mounted in one of the one or more racks adjacent to and spatially separate from a corresponding sample tube, and wherein each sample tube is configured to accept a nucleic-acid containing sample, and wherein each holder comprises a process chamber and one or more receptacles, wherein the one or more receptacles contain one or more reagents suitable for carrying out extraction of nucleic acid from a nucleic-acid containing sample;
a magnetic separator configured to apply a magnetic force to a process chamber of a holder mounted in the rack;
a heater assembly configured to independently heat a process chamber of a holder mounted in the rack; and
a liquid dispenser configured to carry out fluid transfer operations between the plurality of sample tubes and the plurality of holders.
2. The apparatus of claim 1 , further comprising:
a sample identification verifier configured to check an identity of a sample, wherein the sample identification verifier is selected from the group consisting of: an optical character reader, a bar code reader, and a radio frequency tag reader.
3. The apparatus of claim 1 , wherein the liquid dispenser has one or more heads, wherein each head is configured to accept a pipette tip from one or more pipette tips in a holder.
4. The apparatus of claim 1 , further comprising:
electronic circuitry configured to control operation of one or more of the liquid dispenser, the heater assembly, and the magnetic separator.
5. The apparatus of claim 1 , wherein the magnetic separator is configured to move repetitively relative to a process chamber of a holder.
6. The apparatus of claim 1 , wherein the one or more racks comprises two racks.
7. The apparatus of claim 1 , wherein the plurality of sample tubes comprises 12 sample tubes.
8. The apparatus of claim 1 , further comprising a storage area configured to receive and to store nucleic acids, wherein the storage area has a separate location corresponding to each sample tube.
9. The apparatus of claim 1 , further comprising a storage area configured to receive and to store nucleic acids, wherein the storage area comprises a microfluidic cartridge.
10. The apparatus of claim 1 , further comprising a storage area, wherein the storage area comprises a Peltier cooler.
11. The apparatus of claim 1 , further comprising a means for receiving and storing nucleic acid extracted from the nucleic-acid containing samples.
12. The apparatus of claim 1 , wherein each holder comprises a pipette tip sheath configured to surround one or more pipette tips.
13. The apparatus of claim 1 , wherein each holder contains lyophilized sample preparation reagents.
14. The apparatus of claim 1 , wherein one of the one or more reagents comprises: a wash buffer, a release buffer, or a neutralization buffer.
15. The apparatus of claim 1 , wherein the process chamber of each holder has a stellated ridge-pattern on its interior bottom surface.
16. The apparatus of claim 1 , wherein the liquid dispenser is configured to carry out fluid transfer operation on two or more lanes simultaneously, said fluid transfer operations requiring only two-dimensional movement of the liquid dispenser in each lane.
17. A system for preparing a plurality of samples for diagnostic testing, the system comprising:
a rack removably mounted in the system, the rack comprising a plurality of lanes, wherein each lane comprises a slot shaped and sized to receive a holder, and an aperture shaped and sized to receive a sample tube;
a plurality of sample tubes and a plurality of holders, wherein each sample tube is removably mounted in the rack, wherein each holder is removably mounted in the rack adjacent to and spatially separate from a corresponding sample tube, and wherein each sample tube is configured to accept a nucleic-acid containing sample, and wherein each holder comprises a process chamber and one or more receptacles, wherein the one or more receptacles contain one or more reagents suitable for carrying out extraction of nucleic acid from a nucleic-acid containing sample;
an apparatus configured to independently heat a process chamber of a holder mounted in the rack; and
a liquid dispenser configured to carry out fluid transfer operations between the plurality of holders and a microfluidic cartridge removably mounted in the system.
18. The system of claim 17 , wherein the apparatus is configured to apply a magnetic force to a process chamber of a holder mounted in the rack.
19. The system of claim 17 , further comprising the microfluidic cartridge.
20. The system of claim 17 , wherein the liquid dispenser has one or more heads, wherein each head is configured to accept a pipette tip from one or more pipette tips in a holder.