Granted Patent
B2
US 12,397,295 · App. 18/537,233 · Granted Aug 26, 2025
Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
Assignee:
HANDYLAB, INC.
B01L3/502761B01L3/0275B01L3/5027B01L3/52B01L7/52B01L9/06B01L9/527F16K99/0001F16K99/003F16K99/0032F16K99/0044F16K99/0061B01L2200/027B01L2200/10B01L2200/147B01L2200/148B01L2200/16B01L2300/021B01L2300/045B01L2300/06B01L2300/0627B01L2300/0681B01L2300/0816B01L2300/0832B01L2300/0867B01L2300/087B01L2300/0887B01L2300/18B01L2300/1822B01L2300/1827B01L2300/1861B01L2400/0442B01L2400/0481B01L2400/0487B01L2400/0611B01L2400/0677B01L2400/0683F16K2099/0084G01N2035/00881G01N35/026G01N2035/0425G01N2035/0436
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Abstract
The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.
Claims (25)
1. A diagnostic system comprising:
a receiving bay configured to receive a cartridge for performing amplification, wherein the cartridge comprises a cartridge barcode;
a rack configured to receive a plurality of sample tubes and a plurality of reagent holders, wherein each sample tube comprises a sample tube barcode and each reagent holder comprises a reagent tube barcode, wherein the rack comprises a sample tube holder comprising a first rack portion configured to be receive an upper portion of a sample tube of the plurality of sample tubes, the sample tube holder further comprising a second rack portion configured to be receive a lower portion of the sample tube, wherein a middle portion the sample tube is between the upper portion of the sample tube and the lower portion of the sample tube;
a mirror positioned relative to the sample tube holder;
a barcode scanner configured to read the sample tube barcode of the sample tube when the sample tube is received in the sample tube holder, wherein the sample tube barcode is disposed on the middle portion of the sample tube, wherein the barcode scanner has a non-linear field of view from the barcode scanner to the mirror to the middle portion of the sample tube to read the sample tube barcode of the sample tube, wherein the barcode scanner is configured to read the cartridge barcode of the cartridge, wherein the barcode scanner is configured to read the reagent holder barcode of a reagent holder of the plurality of reagent holders; and
a processer, wherein the barcode scanner is configured to communicate details of barcodes that the barcode scanner has detected to the processor.
2. The diagnostic system of claim 1 , wherein sample identifying information is associated with diagnostic results related to sample preparation, extraction, and amplification.
3. The diagnostic system of claim 1 , wherein the reagent holder barcode is positioned upward-facing to facilitate ease of reading by the barcode scanner.
4. The diagnostic system of claim 1 , wherein the reagent holder barcode comprises machine readable information pertaining to an analysis to be performed.
5. The diagnostic system of claim 1 , further comprising a liquid dispenser, wherein the barcode scanner moves with the liquid dispenser.
6. The diagnostic system of claim 1 , further comprising a motorized plate configured to strip pipette tips from a liquid dispenser.
7. The diagnostic system of claim 1 , wherein the cartridge barcode specifies a cartridge type.
8. The diagnostic system of claim 1 , wherein the cartridge barcode specifies a lot number.
9. The diagnostic system of claim 1 , wherein the cartridge barcode specifies an expiry date.
10. The diagnostic system of claim 1 , wherein the sample tube barcode specifies a patient.
11. The diagnostic system of claim 1 , wherein the rack is configured to receive 12 sample tubes and 12 reagent holders.
12. The diagnostic system of claim 1 , wherein the cartridge barcode is applied to the top of the cartridge.
13. The diagnostic system of claim 1 , wherein the presence or absence of the cartridge is detected by reading the cartridge barcode in the receiving bay.
14. The diagnostic system of claim 1 , wherein the presence or absence of the sample tube is detected by reading the sample tube barcode in the rack.
15. The diagnostic system of claim 1 , wherein the rack comprises a registration member configured to ensure the rack is positioned in a single orientation.
16. The diagnostic system of claim 1 , wherein the rack comprises a mechanical key configured to accept the reagent holder in a single orientation.
17. The diagnostic system of claim 1 , wherein the receiving bay is configured to receive the cartridge in a single orientation.
18. The diagnostic system of claim 1 , wherein the plurality of reagent holders are in one-to-one correspondence with the plurality of sample tubes when received in the rack.
19. The diagnostic system of claim 1 , wherein a heat source is positioned in the receiving bay to apply heat to a distinct location of the cartridge when the cartridge is received in the receiving bay.
20. The diagnostic system of claim 1 , wherein the barcode scanner is downward facing.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 22, 2024
From: WILLIAMS, JEFF; WILSON, KERRY; HANDIQUE, KALYAN
To: HANDYLAB, INC.
Reel/Frame 068047/0466 →
Continuity (7)
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