Combination Treatment of Cardiovascular Disease
Disclosed are methods, compositions of matter and cells for treatment of cardiovascular disease through concurrent inhibition of oxidative stress while administration of a cell therapy. The invention also concerts the modulation of oxidative stress for preferential induction of differentiation while concurrently inhibiting inflammatory processes that decrease efficacy of cellular therapy.
1 . A method of amerliorating the effects of myocardial infarction comprising: a) identifying a subject who has suffered from a myocardial infarction b) harvesting mesenchymal stem cells; c) intravenously injecting said mesenchymal stem cells into said subject; and d) administering ascorbic acid or a derivative thereof intravenously to said subject to ameliorate the effects of myocardial infarction.
2 . The method of claim 1 , wherein said ascorbic acid is administered intravenously in an amount of 15-700 g/week.
3 . The method of claim 2 , wherein said ascorbic acid is administered intravenously in an amount of 25 g/day.
4 . The method of claim 1 , wherein said ascorbic acid is intravenously administered to said subject prior to the administration of said mesenchymal stem cells.
5 . The method of claim 1 , further comprising measuring oxidative stress in said subject prior to administration of said mesenchymal stem cells and wherein the ascorbic acid is intravenously administrated at a concentration and frequency based upon said measurement of oxidative stress.
6 . The method of claim 1 , wherein the subject is tested after being administered the ascorbic acid and mesenchymal cells and there is an improvement in ejection fraction and clinical heart failure score.
7 . The method of claim 1 , wherein the subject is tested after being administered the ascorbic acid and mesenchymal stem cells and there is an improvement in ejection fraction.
8 . The method of claim 1 , wherein the subject is tested after being administered the ascorbic acid and mesenchymal stem cells and there is an improvement in clinical heart failure score.