Reactive oxygen species (ROS)-responsive compositions and methods thereof
A reactive oxygen species savaging emulsion; the emulsion comprising an injectable pharmaceutically acceptable composition and a polymeric poly(propylene sulfide) microsphere for targeted delivery to a site with elevated reactive oxygen species. In embodiments of the present invention, the microsphere is loaded with a biologically active agent.
1. A reactive oxygen species scavenging emulsion, the emulsion comprising an injectable pharmaceutically acceptable composition and a polymeric microsphere for targeted delivery to a site with elevated reactive oxygen species;
wherein the polymer of the polymeric microsphere consists of poly(propylene sulfide); and
wherein the microsphere has a diameter of at least 0.5 μm.
2. The emulsion of claim 1 , wherein the microsphere is loaded with a biologically active agent.
3. The emulsion of claim 2 , wherein the biologically active agent is at least one of an enzyme, organic catalyst, antibiotic, antioxidant, anti-reactive oxygen species (ROS) agent, anti-inflammatory, protein, glycoprotein, peptide, polyamino acid, antibody, epitopes of antibodies, nucleic acid, steroidal molecule, antiviral, antirejection agent, immunosuppressant, cytokine, carbohydrate, pharmaceutical, cell, virus, single chain fragment, siRNA, miRNA against the p53/MAP kinase pathway, virus vector, prion, anti-proliferative agents, anti-migratory agents, biologically active polymers, and combinations thereof.
4. The emulsion of claim 2 , wherein the biologically active agent is curcumin.
5. The emulsion of claim 2 , wherein the biologically active agent is anti-reactive oxygen species (ROS) agents.
6. The emulsion of claim 2 , wherein the biologically active agent is superoxide dismutase mimetic 4-hydroxy-TEMPO benzoate.
7. The emulsion of claim 2 , wherein the biologically active agent is an NF-κB pathway inhibitor.
8. The emulsion of claim 7 , wherein the NF-κB pathway inhibitor is TPCA-1.
9. The emulsion of claim 2 , wherein the biologically active agent is hydrophobic.
10. The emulsion of claim 1 , wherein the emulsion comprises at least one of an antioxidant, buffer, bacteriostat, bacterial antibiotic, or a combination thereof.
11. The emulsion of claim 1 , wherein the microsphere is about 0.5 μm to about 1.5 μm in diameter.
12. The emulsion of claim 1 , wherein the microsphere is about 1 μm to about 5 μm in diameter.
13. A method of treating inflammation-related pathologies in a patient in need thereof, comprising:
administering an inflammation-reducing effective amount of an emulsion, the emulsion comprising an injectable pharmaceutically acceptable composition and a polymeric microsphere for targeted delivery to a site with elevated reactive oxygen species, wherein the polymer of the polymeric microsphere consists of poly(propylene sulfide);
wherein the microsphere has a diameter of at least 0.5 μm and
wherein the microsphere is loaded with a biologically active agent.
14. The method of claim 13 , further comprising, prior to the administration step, identifying an inflammation site in the patient, and locally injecting the emulsion at the inflammation site.
15. The method of claim 13 , wherein the biologically active agent is at least one of an enzyme, organic catalyst, antibiotic, antioxidant, anti-reactive oxygen species (ROS) agent, anti-inflammatory, protein, glycoprotein, peptide, polyamino acid, antibody, epitopes of antibodies, nucleic acid, steroidal molecule, antiviral, antirejection agent, immunosuppressant, cytokine, carbohydrate, pharmaceutical, cell, virus, single chain fragment, siRNA, miRNA against the p53/MAP kinase pathway, virus vector, prion, anti-proliferative agents, anti-migratory agents, biologically active polymers, and combinations thereof.
16. The method of claim 13 , wherein the biologically active agent is curcumin, TPCA-1, superoxide dismutase mimetic 4-hydroxy-TEMPO benzoate.
17. A method of locally delivering an anti-inflammatory agent to a subject in need thereof, comprising:
identifying a site of oxidative stress and/or identifying an inflammation site;
administering a reactive oxygen species (ROS) scavenging effective amount of the emulsion of claim 1 to the site to locally treat the inflammation;
wherein the microsphere is loaded with a biologically active agent.
18. The method of claim 17 , wherein the emulsion is loaded with curcumin.
19. A method of locally delivering an anti-peripheral artery disease agent to a subject in need thereof, comprising:
identifying an inflammation site;
administering a reactive oxygen species (ROS) scavenging effective amount of the emulsion of claim 1 to the site to locally treat peripheral artery disease;
wherein the microsphere is loaded with a biologically active agent.
20. The method of claim 19 , wherein the emulsion is loaded with curcumin.
21. A method of locally treating osteoarthritis to a subject in need thereof, comprising:
identifying an osteoarthritis site;
administering an effective cartilage damage reducing amount of the emulsion of claim 1 to the site to locally treat the osteoarthritis;
wherein the microsphere is loaded with a biologically active agent.
22. The method of claim 21 , wherein the emulsion is loaded with TPCA-1.