Immunogenic compositions containing
Immunogenic compositions containing Escherichia coli 0157:H7 flagella including fusion proteins and methods using the immunogenic compositions are disclosed. Inducing an immune response in an animal to Escherichia coli O157:H7 flagella will result in prevention of colonization by Escherichia coli O157:H7 in the animal or a reduction in the amount of Escherichia coli O157:H7 infecting the animal. The immune composition will prevent or reduce the attachment of Escherichia coli 0157:H7 to cells within the animal.
1. A method for generating a mucosal and/or IgA response in a subject in need thereof, said method comprising the parenteral administration of Escherichia coli H7 or a Toll-like receptor 5 (TLR5) activating fragment thereof.
2. The method of claim 1 , wherein the subject is an agricultural animal.
3. The method of claim 2 , wherein the agricultural animal is a ruminant animal.
4. The method of claim 1 , wherein the H7 or TLR5 activating fragment thereof is derived from Escherichia coli O157:H7.
5. The method of claim 1 , wherein the H7 or TLR5 activating fragment thereof comprises an amino acid sequence at least 97% homologous to SEQ ID NO: 2.
6. The method of claim 1 , wherein the H7 or TLR5 activating fragment thereof comprises the amino acid sequence of SEQ ID NO: 2.
7. The method of claim 1 , wherein the H7 or TLR5 activating fragment thereof is encoded by the nucleic acid sequence of SEQ ID NO: 1.
8. The method of claim 1 , wherein the method further comprises administering an adjuvant.