Methods for improving recombinant protein expression
Materials and methods are provided which allowed for increased expression of a transfected gene of interest in a recombinant host cell.
1. An expression vector comprising Chinese hamster elongation factor 1 (CHEF1) regulatory DNA, a first polynucleotide encoding a gene of interest, and a second polynucleotide comprising a codon deoptimized selectable marker gene sequence encoding a selectable marker protein having an amino acid sequence identical to wild-type, wherein said first polynucleotide and said second polynucleotide are under transcriptional control of distinct promoters, and wherein the second polynucleotide comprises SEQ ID NO:3 or SEQ ID NO:4.
2. An isolated host cell transformed or transfected with the expression vector according to claim 1 .
3. The host cell of claim 2 , wherein the host cell is a Chinese hamster cell.
4. The host cell of claim 3 , wherein the host cell is a Chinese hamster ovary (CHO) cell.
5. The host cell of claim 4 , wherein the host cell is a CHO DG44 cell.