Dual targeting drug carrier and application thereof
A dual targeting drug carrier is provided. The dual targeting drug carrier comprises a first targeting molecule and a second targeting molecule, wherein the targeting molecule comprises peptide, protein or antibody. The targeting molecule can bind to specific receptors, proteins, or glycoproteins to recognize the specific tumor cells, tissues, or organs. The dual targeting drug carriers are further conjugated with imaging agents, radioactive molecules (radiopharmaceuticals, isotopes, or chemotherapeutic drugs) or nanoparticles to form a conjugate.
1. A dual targeting drug carrier consisting of a first targeting molecule and a second targeting molecule,
wherein the first targeting molecule is selected from the group consisting of Arg-Gly-Asp (RGD) and RGD4C, and the second targeting molecule is selected from the group consisting of epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF),
wherein the first targeting molecule and the second targeting molecule are selected from the group consisting of RGD-EGF (SEQ ID NO: 2), RGD4C-EGF (SEQ ID NO: 3), RGD-VEGF (SEQ ID NO: 7), and RGD4C-VEGF (SEQ ID NO: 8).
2. The dual targeting drug carrier according to claim 1 , wherein the first and second targeting molecules specifically bind to tumor cells or vascular endothelial cells in tumor microenvironment.
3. A radio-labeled fusion protein, comprising the dual targeting drug carrier of claim 1 and a radioactive nuclide.
4. The radio-labeled fusion protein according to claim 3 , wherein the dual targeting drug carrier is linked to the radioactive nuclide by a metal chelator.
5. The radio-labeled fusion protein according to claim 4 , wherein the metal chelator is selected from a group consisting of DTPA, NOTA, DOTA, and derivate thereof.