Methods for identifying agents useful for modulating the expression and aggregation of CAG-expanded gene product in cells
This invention provides a method for modulating the expression of a first gene in a cell wherein the first gene is one containing more than 36 CAG trinucleotide repeats and encoding a protein that form polyglutamine-mediated protein aggregation. Suppression of the first gene is achieved by reducing the expression of SPT4 gene or SUPT4H gene. It can also be achieved by inhibiting the formation of a Spt4/Spt5 complex or a Supt4h/Supt5h complex. Also provided is a method for identifying an agent useful for modulating the expression and aggregation of CAG-expanded gene product, or treating a polyglutamine disease such as Huntington's disease.
1. A method for identifying a compound useful for treating a polyglutamine disease, said method comprising:
providing a sample from a subject of said polyglutamine disease, said sample having a first gene containing CAG repeats with more than 36 repeats; and
screening a plurality of test compounds to identify one having an inhibitory activity capable of disrupting formation of an Spt4/Spt5 protein complex or an Supt4h/Supt5h protein complex,
wherein said compound is an antibody, a small reagent, or a peptide, and said compound can modulate the expression of the first gene.