Cobalamin acquisition protein and use thereof
The present invention relates to a cobalamin acquisition protein, compositions containing the cobalamin acquisition protein, and the use of such compositions.
1. A recombinant expression vector construct comprising a heterologous nucleic acid encoding a protein that binds Vitamin B 12 and comprising an amino acid sequence having at least 90% identity with SEQ ID NO: 2.
2. The construct of claim 1 , wherein the nucleic acid is operatively associated with an inducible promoter.
3. The construct of claim 1 , wherein the nucleic acid is operatively associated with a constitutive promoter.
4. The construct of claim 1 , wherein the protein binds Vitamin B 12 with an affinity of less than 10 −5 M.
5. The construct of claim 1 , wherein the protein binds Vitamin B 12 with an affinity from about 10 −5 M to about 10 −12 M.