Multi-sample laser-scatter measurement instrument with incubation feature and systems for using the same
An optical measurement instrument is an integrated instrument that includes an optical cavity with a light source, a sample cuvette, and an optical sensor. The light source and sensor are on a bench that is on a translational or rotational mechanical platform such that optical beam can be moved to multiple sample containers. The instrument can be used for taking measurements of organism concentration in multiple samples as a production tool for microbiology. Preferably, the instrument holds multiple, individually-loaded, independent fluid samples and determines bacteria concentration via a forward-scattering signal. The instrument can incorporate onboard incubation to promote bacterial growth in the samples during the test. In another aspect, the instrument can be a part of a network for medical diagnostic testing data where data is stored in a manner that is inherently untainted by patient identifiable information.
1. An optical measuring instrument for determining a concentration of bacteria in a plurality of fluid samples, comprising:
a housing with a substantially light-tight enclosure;
a single cuvette assembly having a plurality of internals walls defining a plurality of individual internal fluid containers within the single cuvette assembly, each of the fluid containers holding a corresponding one of the plurality of fluid samples, each of the fluid containers having an input window and an output window, the single cuvette assembly including a code on an exterior surface thereof, the code providing information for a protocol for a test to be conducted by the optical measuring instrument on the fluid samples within the internal fluid containers within the single cuvette assembly;
a platform structure located within the housing, the single cuvette assembly being registered on the platform structure;
a laser located on an optical bench within the housing for providing an input beam for transmission into the input windows of the fluid containers and though the corresponding fluid samples, the input beam creating a forward-scatter signal associated with the concentration of bacteria, the laser periodically providing the input beam into each one of the fluid samples such that the multiple forward-scatter symbols are detected over a period of time for each of the fluid samples;
at least one sensor located on the optical bench within the housing for detecting the forward-scatter signal exiting from the output windows;
a code sensor for reading the information from the code on the single cuvette assembly;
a heating element within the housing to maintain the fluid samples at a desired temperature to encourage bacterial growth in the fluid samples over a period of time, the desired temperature being defined by the protocol indicated by the code; and
wherein the single cuvette assembly with the fluid containers is held in a fixed position and the optical bench undergoes translational movement so that the input beam from the laser sequentially addresses each of the plurality of fluid samples.
2. The optical measuring instrument of claim 1 , wherein the heating element is part of an incubation system that further includes at least one temperature sensor for providing thermostatic control.
3. The optical measuring instrument of claim 1 , wherein the single cuvette assembly with the individual fluid containers can be loaded through a door on the housing.
4. The optical measuring instrument of claim 1 , wherein the number of multiple scatter signals for each of fluid samples is greater than twenty within a 2-hour test period.
5. The optical measuring instrument of claim 1 , further including a display device coupled to the optical measuring instrument, the display device for displaying a bacterial growth curve for at least some of the fluid samples.
6. The optical measuring instrument of claim 5 , further including an alarm for indicating when a bacterial growth curve exceeds a certain slope or the forward-scatter signal exceeds a certain value.
7. The optical measuring instrument of claim 1 , wherein the single cuvette assembly includes a filter, the fluid sample passing through the filter before transmission of the input beam into the input windows.
8. An optical measurement system for use in optically measuring bacteria within a fluid sample, comprising:
a light source for producing an input beam;
a removable single cuvette assembly having a plurality of internal walls that define a plurality of internal fluid containers within the single cuvette assembly, the single cuvette assembly having individual windows associated with each of the plurality of fluid containers, the single cuvette assembly remaining in a fixed position within the system during operation, the single cuvette assembly including a code on an exterior surface thereof for providing information for a protocol for a test to be conducted by the optical measuring instrument on the fluid samples within the internal fluid containers of the single cuvette assembly;
a sensor for receiving a forward-scatter signal caused by the input beam passing through each of the plurality of fluid containers containing the fluid sample with the bacteria;
a heating system that permits a controlled incubation temperature for the fluid sample, the controlled incubation temperature during the test being indicated by information from the code; and
a moveable optical bench undergoing translational movement, the light source and the sensor being mounted on the movable optical bench, the translational movement of the optical bench permitting the fluid samples within each of the plurality of fluid containers within the fixed single cuvette assembly to be sequentially placed into a path of the input beam such that the sensor receives multiple forward-scatter signals for each of the plurality of fluid samples over a period of time.
9. The optical measuring instrument of claim 1 , wherein the code further provides information regarding a frequency of the optical measurements duration of the test.