MICROPLATES FOR MAGNETIC 3D CULTURE
Devices for magnetic 3d culture are described including magnetic lids/bases for single Petri plates and adjustable height cap for same. Similar devices for multi-magnet culture plates wherein multiwell plates have all adjacent magnets orientated in the opposite polarity, and methods of making same.
1 . A magnetic culture plate, comprising
a) a cell culture vessel and a cap for said vessel,
b) said cap having a lip around an outer circumference thereof and being shaped to fit over or under said cell culture vessel,
c) said cap having a depression into which a magnet is affixed, thus holding said magnet over said culture vessel when said cap is in place over said vessel.
2 . The magnetic culture plate of claim 1 , wherein said vessel has a plurality of wells, and said cap has a plurality of depressions, in each of which a magnet is affixed, such that every magnet is orientated in an opposite orientation to an adjacent magnet.
3 . The magnetic culture plate of claim 1 or 2 , said magnet being adhered to a bottom of said depression.
4 . The magnetic culture plate of claim 1 or 2 , said magnet being snap fit into said depression.
5 . A multiwell cell culture vessel, comprising a vessel having plurality of wells in an array, a cap covering vessel and said plurality of wells, said cap having a plurality of magnets in an array, each magnet affixed to a depression in said cap or on a post on said cap, such that each of said wells has a magnet over said well when said cap is in place over said vessel.
6 . The multiwell magnetic cell culture vessel of claim 6 , each adjacent magnet being affixed in opposite polarity.
7 . A magnetic cap for a microplate, comprising a cap sized to cover a standard ANSI-SLAS microplate having a plurality of wells in an array, said cap having lip around a circumference thereof and a plurality of magnets in an array, each magnet affixed to a depression in said cap or on a post on said cap, such that each magnet sits over a well when said cap is in place over or under said microplate and such that each adjacent each adjacent magnet is affixed in opposite polarity.
8 . The magnetic cap of claim 8 , having 6 magnets of 20-100 lbs pull force, 10000-15000 Gauss Br max and 30-60 MGOe Bh max .
9 . The magnetic cap of claim 8 , having 12 magnets of 2-20 lbs pull force, 10000-15000 Gauss Br max and 30-60 MGOe Bh max .
10 . The magnetic cap of claim 8 , having 24 magnets of 2-10 lbs pull force, 10000-15000 Gauss Br max and 30-60 MGOe Bh max .
11 . The magnetic cap of claim 8 , having 96 magnets of 0.5-2 lbs pull force, 10000-15000 Gauss Br max and 30-60 MGOe Bh max .
12 . The magnetic cap of claim 8 , having 384 magnets of 0.05-1 lbs pull force, 10000-15000 Gauss Br max and 30-60 MGOe Bh max .
13 . The magnetic cap of claim 8 , having the dimensions of FIG. 7, 8, 9, 10, 11 or 12 .
14 . The magnetic cap of claim 8 , said magnets having the dimensions and strengths of FIG. 13 .
15 . A method of making a magnetic driver for a standard microtiter plate, said method comprising:
a) obtaining a cap having a circumferential lip that fits both over and under a separate microtiter plate having a plurality of wells; and
b) affixing a plurality of magnets to said cap such that every well has a magnet over said well when said cap is in place over or under said microtiter plate, and such that every magnet has a polarity opposite each adjacent magnet.
16 . The method of claim 16 , wherein said affixing step is snap-fitting each magnet to a depression in an upper surface of said cap.
17 . The method of claim 16 , wherein said affixing step is adhering each magnet to a depression in an upper surface of said cap.
18 . The method of claim 16 , wherein said affixing step is fitting a ring magnet over a post on a surface of said cap.
19 . A method of 3D cell culture, comprising:
a) incubating one or more cell types in a solution of iron oxide nanoparticles until said cell types contain about 30-150 pg/cell of iron oxide;
b) suspending said cell types containing said iron oxide in a culture medium;
c) aliquoting samples of said suspended cell types to one or more wells of a microplate;
d) placing the magnetic cap of claim 8 above or below said microplate;
e) incubating said microplate until a 3D culture of cells or desired 3D culture printed shape is formed; and
f) incubating said microplate under the influence of the magnetic field for the duration of the 3D culture.