Production and composition of fructose syrup
View Patent ↗Disclosed herein are aqueous compositions comprising fructose and methods of production thereof. A method for producing an aqueous composition comprising fructose can comprise, for example, conducting an enzymatic reaction by contacting water, sucrose, and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan having at least 30% alpha-1,3-linkages. A soluble fraction produced by such a reaction comprises at least about 55% fructose on a dry weight basis, and can be separated from insoluble poly alpha-1,3-glucan product(s), thereby providing an aqueous composition comprising fructose.
1. A method for producing fructose, said method comprising:
contacting water, sucrose, and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan having at least 30% alpha-1,3-linkages to produce a soluble fraction and an insoluble fraction,
wherein the insoluble fraction comprises said poly alpha-1,3-glucan,
wherein the soluble fraction comprises at least 55% fructose on a dry weight basis, and
wherein the method does not comprise a process step that increases the content of fructose relative to the content of other saccharides in the soluble fraction.
2. The method of claim 1 , wherein the glucosyltransferase enzyme synthesizes poly alpha-1,3-glucan having at least 95% alpha-1,3-linkages.
3. The method of claim 1 , wherein the soluble fraction further comprises soluble oligosaccharides with a degree of polymerization (DP) of 2 to about 15.
4. The method of claim 3 , wherein the soluble fraction comprises less than about 30% of said soluble oligosaccharides on a dry weight basis.
5. The method of claim 1 , wherein the soluble fraction comprises at least about 75% fructose on a dry weight basis.
6. The method of claim 1 , wherein the sucrose concentration of the soluble fraction is less than 2 g/L.
7. The method of claim 1 , further comprising separating the soluble fraction from the insoluble fraction.