Anti-5T4 antibodies and uses thereof
Anti-5T4 antibodies, anti-5T4 antibody/drug conjugates, and methods for preparing and using the same.
1. An isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence of nucleotides 58-414 of SEQ ID NO: 1;
(b) a nucleotide sequence set forth as any one of SEQ ID NOs: 48, 50, 53, or 55;
(c) a nucleotide sequence encoding a heavy chain variable region having an amino acid sequence set forth as any one of SEQ ID NOs: 49, 51, 52, 54, or 56;
(d) a nucleotide sequence of nucleotides 55-405 of SEQ ID NO: 5;
(e) a nucleotide sequence encoding a heavy chain variable region having an amino acid sequence set forth as any one of SEQ ID NOs: 77 or 78;
(f) a nucleotide sequence of nucleotides 58-423 of SEQ ID NO: 9; and
(g) a nucleotide sequence encoding a heavy chain variable region having an amino acid sequence set forth as any one of SEQ ID NOs: 81 or 82.
2. An isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence of nucleotides 61-381 of SEQ ID NO: 3;
(b) a nucleotide sequence set forth as any one of SEQ ID NOs: 57, 59, 61, 63, 65, 67, 69, 71, 73, or 75;
(c) a nucleotide sequence encoding a light chain variable region having an amino acid sequence set forth as any one of SEQ ID NOs: 58, 60, 62, 64, 66, 68, 70, 72, 74, or 76,
(d) a nucleotide sequence of nucleotides 67-390 of SEQ ID NO: 7;
(e) a nucleotide sequence encoding a light chain variable region having an amino acid sequence set forth as any one of SEQ ID NOs: 79 or 80;
(f) a nucleotide sequence of nucleotides 61-381 of SEQ ID NO: 11; and
(g) a nucleotide sequence encoding a light chain variable region having an amino acid sequence set forth as any one of SEQ ID NOs: 83 or 84.
3. The isolated nucleic acid of claim 1 , further comprising a nucleotide sequence encoding a human heavy chain constant region.
4. The isolated nucleic acid of claim 3 , wherein the human heavy chain constant region is a human IgG4 heavy chain constant region.
5. The isolated nucleic acid of claim 4 , wherein the human IgG4 heavy chain constant region has an amino acid sequence set forth as SEQ ID NO: 45.
6. The isolated nucleic acid of claim 3 , wherein the human heavy chain constant region is a human IgG1 heavy chain constant region.
7. The isolated nucleic acid of claim 6 , wherein the human IgG1 heavy chain constant region has an amino acid sequence set forth as SEQ ID NO: 46.
8. The isolated nucleic of claim 2 , further comprising a nucleotide sequence encoding a human light chain constant region.
9. The isolated nucleic acid of claim 8 , wherein the human light chain constant region is a human kappa light chain constant region.
10. The isolated nucleic acid of claim 9 , wherein human kappa light chain constant region has an amino acid sequence set forth as SEQ ID NO: 47.
11. A vector comprising the isolated nucleic acid of claim 1 .
12. A vector comprising the isolated nucleic acid of claim 2 .
13. The vector of claim 11 , further comprising the isolated nucleic acid of claim 2 .
14. The vector of claim 11 , comprising a nucleotide sequence set forth as SEQ ID NO: 53.
15. The vector of claim 12 , comprising a nucleotide sequence set forth as SEQ ID NO: 69.
16. The vector of claim 11 , further comprising a nucleotide sequence set forth as SEQ ID NO: 69.
17. The vector of claim 14 , further comprising a nucleotide sequence encoding a human IgG1 heavy chain constant region having an amino acid sequence set forth as SEQ ID NO: 46.
18. The vector of claim 15 , further comprising a nucleotide sequence encoding a human kappa light chain constant region having an amino acid sequence set forth as SEQ ID NO: 47.
19. The vector of claim 17 , further comprising a nucleotide sequence encoding a human kappa light chain constant region having an amino acid sequence set forth as SEQ ID NO: 47.
20. A vector comprising:
(a) an isolated nucleic acid comprising a nucleotide sequence encoding a heavy chain variable region having an amino acid sequence set forth as SEQ ID NO: 54;
(b) an isolated nucleic acid comprising a nucleotide sequence encoding a human IgG1 heavy chain constant region having an amino acid sequence set forth as SEQ ID NO: 46;
(c) an isolated nucleic acid comprising a nucleotide sequence encoding a light chain variable region having an amino acid sequence set forth as SEQ ID NO: 70; and
(d) an isolated nucleic acid comprising a nucleotide sequence encoding a human kappa light chain constant region having an amino acid sequence set forth as SEQ ID NO: 47.