Process for producing a particulate composition comprising crystalline trehalose dihydrate
A process for enabling the production of a particulate composition containing crystalline trehalose dihydrate is provided. Including allowing an α-glycosyltrehalose-forming enzyme to act on liquefied starch derived from a microorganism of the genus Arthrobacter and a trehalose-releasing enzyme derived from a microorganism of the genus Arthrobacter along with a starch debranching enzyme and a cyclomaltodextrin glucanotransferase; allowing glucoamylase to act on the resulting mixture to obtain a saccharide solution containing α,α-trehalose; precipitating crystalline α,α-trehalose dihydrate from the above saccharide solution; collecting the precipitated crystalline α,α-trehalose dihydrate by a centrifuge; and ageing and drying the collected crystals. Cyclomaltodextrin glucanotransferase derived from a microorganism of the genus Paenibacillus or a mutant enzyme thereof is used to increase the α,α-trehalose content in the saccharide solution to over 86.0% by weight, on a dry solid basis, without passing through a fractionation step by column chromatography.
1. A particulate composition comprising crystalline α-α-trehalose dihydrate, in which:
(1) said particulate composition has an α-α-trehalose purity of 99.2% by weight or more but not more than 99.7% by weight, on a dry solid basis;
(2) said particulate composition has a degree of crystallinity for crystalline α-α-trehalose dihydrate of 91.0% or higher but not higher than 96.8%,
when calculated based on its powder X-ray diffraction profile; and
(3) said particulate composition contains particles with a particle size of at least 53 μm but smaller than 425 μm in an amount of 70% by weight or more based on the particulate composition as a whole, and particles with a particle size of at least 53 μm but smaller than 300 μm in an amount of 50% by weight or more based on the particulate composition as a whole.
2. The particulate composition comprising crystalline α,α-trehalose dihydrate of claim 1 , which has a reducing power of the whole particulate composition being not more than 0.5% by weight.
3. The particulate composition comprising crystalline α,α-trehalose dihydrate of claim 1 or claim 2 ,
wherein said degree of crystallinity for crystalline α, α-trehalose dihydrate is calculated by the following Formula [3]:
Degree of crystallinity (%)={( Hs−H 0 )/( H 100 −H 0 )}×100 <Formula [3]>
H 100 : An analytical value for a degree of crystallinity, determined based on the powder X-ray diffraction profile for a powdered standard sample containing crystalline trehalose dihydrate, where the powdered standard sample consists substantially of crystalline trehalose dihydrate;
H 0 : An analytical value for a degree of crystallinity, determined based on the powder X-ray diffraction profile for a powdered standard sample containing trehalose, where the powdered standard sample consists substantially of an amorphous form of trehalose;
Hs: An analytical value for a degree of crystallinity, determined based on the powder X-ray diffraction profile for, as a test sample, a powder containing trehalose.