Thermophilic and thermoacidophilic glycosylation genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for glycosylating and/or post-translationally modifying proteins using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.
1. A vector comprising:
a first polynucleotide encoding a polypeptide comprising at least 90% sequence identity to SEQ ID NO:205; and
a second polynucleotide heterologous to the first polynucleotide.
2. The vector of claim 1 , wherein the first polynucleotide comprises SEQ ID NO:206.
3. The vector of claim 1 , wherein the polypeptide encoded by the first polynucleotide comprises SEQ ID NO:205 and has enzyme activity as a glycosyltransferase at a temperature at or above about 35 degrees Celsius.