Oriented loading systems and method for orienting a particle loaded in a well
An oriented loading system is provided. The oriented loading system includes a substrate, a plurality of wells formed in the substrate, each well having a bottom and sidewalls, a plurality of particles loaded in the wells, wherein the particle comprises a core structure and an inner layer comprising magnetic material partially covering the core structure such that a part of the core structure uncovered by the inner layer is exposed, and a metal layer comprising magnetic material deposited partially in the sidewalls of the wells, wherein the inner layer is attracted by the metal layer such that the exposed core structure is oriented towards the bottom of the well or the inner layer is oriented towards the bottom of the well.
1. An oriented loading system, comprising:
a substrate;
a plurality of wells formed in the substrate, each well having a depth, a bottom and sidewalls, the sidewalls having an upper part and a lower part;
a plurality of particles loaded in the wells, wherein each of the plurality of particles comprises a core structure and an inner layer comprising magnetic material partially covering the core structure, or the inner layer is further covered by an outer layer, wherein a part of the core structure uncovered by the inner layer is exposed or is further covered by a coating layer; and
a metal layer comprising magnetic material deposited partially in the sidewalls of the wells, wherein
the inner layer is attracted by the metal layer such that the exposed core structure or the coating layer, or the inner layer or the outer layer becomes a bottom side of the particle oriented towards the bottom of the well, wherein the bottom side of the particle is modified by an electrically charged molecule, and the bottom of the well is modified by an electrically charged molecule, wherein the electrically charged molecule on the bottom side of the particle comprises opposite charge to the electrically charged molecule on the bottom of the well, wherein
when the metal layer is deposited in the upper part of the sidewalls, the exposed core structure or the coating layer which is modified by the electrically charged molecule is oriented towards the bottom of the well, or the inner layer or the outer layer which is modified by the electrically charged molecule is oriented towards the bottom of the well, or
when the metal layer is deposited in the lower part of the sidewalls, the inner layer or the outer layer which is modified by the electrically charged molecule is oriented towards the bottom of the well.
2. The oriented loading system as claimed in claim 1 , wherein the outer layer and the coating layer comprise non-magnetic material, and the coating layer is different from the outer layer.
3. The oriented loading system as claimed in claim 2 , wherein the non-magnetic material is polystyrene, hydrogel, silica, alumina, diamond, or gold, silver, copper or an alloy thereof.
4. The oriented loading system as claimed in claim 1 , wherein the core structure comprises polystyrene, hydrogel, silica, alumina, diamond, or gold, silver, copper or an alloy thereof.
5. The oriented loading system as claimed in claim 1 , wherein the magnetic material of the inner layer and the magnetic material of the metal layer are independently selected from the group consisting of iron, cobalt, nickel and an alloy thereof.
6. The oriented loading system as claimed in claim 1 , wherein the inner layer or the outer layer has a thickness of 5-1,000 nm.
7. The oriented loading system as claimed in claim 1 , wherein the particle has a diameter of 50 nm-10 μm.
8. The oriented loading system as claimed in claim 1 , wherein the well has a width which is 1-3 times the diameter of the particle.
9. The oriented loading system as claimed in claim 1 , wherein the well has a depth which is 0.1-5 times the diameter of the particle.
10. The oriented loading system as claimed in claim 1 , wherein the particles further comprise neutrally charged molecules modified thereon.
11. The oriented loading system as claimed in claim 1 , wherein the electrically charged molecules on the bottom of the wells comprise a zwitterionic molecule.