Blood analysis method, control device, and blood cell analyzer
The present disclosure provided a blood cell analyzer, a control device and a blood analysis method thereof. In the method, a first reagent is mixed with a sample to obtain a first testing sample, and then a second reagent is mixed with the first testing sample for a further reaction to get a second testing sample for basophil classification and/or HGB measurement. A blood sample may be tested in one reaction cell through time-division multiplexing technology to obtain four groups leukocytes classification result and HGB result by single detection channel. Thus, the structure of the analyzer may be greatly simplified on the premise of guaranteeing the performance of the analyzer, the size and cost of the analyzer may reduce and a performance-price ratio of the analyzer may increase.
1. A blood analyzer, said blood analyzer comprising:
a reaction cell, where a testing sample is prepared from a blood sample;
a detection apparatus, which detects the testing sample for leukocyte detection; a sample injecting apparatus, which injects the blood sample into the reaction cell;
a reagent injecting apparatus, which injects a reagent into the reaction cell; a transferring apparatus, which transfers the testing sample from the reaction cell to the detection apparatus;
a control device, which couples to the sample injecting apparatus, the reagent injecting apparatus and the transferring apparatus respectively;
wherein
the control device controls the sample injecting apparatus to inject a blood sample into the reaction cell, controls the reagent injecting apparatus to inject a first reagent for leukocyte classification into the reaction cell, wherein the first reagent lyses erythrocytes in the blood sample and reacts with leukocytes in the blood sample to obtain a first testing sample;
the control device controls the transferring apparatus to transfer a part of the first testing sample from the reaction cell to the detection apparatus for a leukocyte classification detection after said reaction between the blood sample and the first reagent is finished;
the control device controls the reagent injecting apparatus to inject a second reagent into the reaction cell having a remainder of the first testing sample after the part of the first testing sample is transferred, wherein the remainder of the first testing sample reacts with the second reagent which further reacts with leukocytes to obtain a second testing sample; and
the control device controls the transferring apparatus to transfer the second testing sample to the detection apparatus or a hemoglobin measurement apparatus after the leukocyte classification detection is finished.
2. The blood analyzer according to claim 1 , wherein the transferring apparatus comprises a syringe which couples to the control device and a transfer pipeline which connects to the syringe; the syringe, a testing sample outlet of the reaction cell and an inlet of the detection apparatus are connected by the transfer pipeline, and the testing sample outlet has a first controllable valve for controlling “on-off” of the outlet.
3. The blood analyzer according to claim 2 , said blood analyzer further comprising a cleaning pipeline, wherein
a first end of the cleaning pipeline connects to the transfer pipeline and the testing sample outlet of the reaction cell, and a second end of the cleaning pipeline connects to a waste collecting apparatus, and the cleaning pipeline has a second controllable valve for controlling “on-off” of the cleaning pipeline;
the control device couples to the first controllable valve and the second controllable valve respectively, which controls the syringe to transfer the part of the first testing sample which is prepared by the blood sample and the first reagent from the reaction cell to the detection apparatus after the reaction between the blood sample and the first reagent is finished, controls the first controllable valve as “on” and the second controllable valve as “off” during the part of the first testing sample is drawn, and controls the first controllable valve off and the second controllable valve as “off” after drawing of the part of the first testing sample is finished;
the control device controls the first controllable valve as “off” and the second controllable valve as “on” after the leukocyte classification detection is finished, and controls the syringe to draw a cleaning solution to clean the transfer pipeline and the detection apparatus and discharge the cleaning solution from the cleaning pipeline;
the control device controls the first controllable valve as “on” and the second controllable valve as “off” after both the cleaning and the reaction between the remainder of the first testing sample and the second reagent are finished, and controls the syringe to transfer a part of the second testing sample through the transfer pipeline from the reaction cell to the detection apparatus; and
the control device controls the first controllable valve as “off” and the second controllable valve as “off” after the drawing of the part of the second testing sample is finished.
4. The blood analyzer according to claim 2 , wherein said syringe comprises a first syringe and a second syringe, the sample injecting apparatus comprises a sample probe, and the transfer pipeline comprising:
a sample acquiring pipeline, which has a first end and a second end, and the first end of the sample acquiring pipeline connects to the sample probe;
a first sample preparing pipeline, which has a first end and a second end, and the first end of the first sample preparing pipeline connects to the testing sample outlet of the reaction cell;
a second sample preparing pipeline, which has a first end and a second end, and the first end of the second sample preparing pipeline connects to the second end of the first sample preparing pipeline and the inlet of the detection apparatus;
a first connecting pipeline, which has a first end and a second end, and the first end of the first connecting pipeline connects to the first syringe, the second end of the first connecting pipeline connects to an inlet for a diluent input;
a second connecting pipeline, which has a first end and a second end, and the first end of the second connecting pipeline connects to the second syringe, the second end of the second connecting pipeline connects to the second end of the sample acquiring pipeline and the second end of the second sample preparing pipeline respectively by a third controllable valve;
an auxiliary pushing pipeline, which has a first end, a second end and a fourth controllable valve, and the first end of the auxiliary pushing pipeline connects to the second connecting pipeline, the second end of the auxiliary pushing pipeline connects to the first connecting pipeline; and
a sheath fluid pipeline, which has a first end, a second end and a fifth controllable valve, and the first end of the sheath fluid pipeline connects to the first connecting pipeline, the second end of the sheath fluid pipeline connects to the detection apparatus.
5. The blood analyzer according to claim 1 , said blood analyzer further comprising a hemoglobin measurement apparatus which couples to the control device, and being controlled to measure the second testing sample for hemoglobin measurement while the remainder of first testing sample reacts with the second reagent.