INCREASED T-CELL TUMOR INFILTRATION AND ERADICATION OF METASTASES BY MUTANT LIGHT
Mutant LIGHT expressed in a tumor environment elicited high levels of chemokines and adhesion molecules, accompanied by massive infiltration of naïve T lymphocytes. Methods and compositions to elicit immune responses against tumors including tumor volume reduction and eradication of metastasis using mutant LIGHT are disclosed.
1 .- 39 . (canceled)
40 . A method of inducing tumor-specific T-cell generation to reduce or control metastasis, the method comprising:
(a) introducing a mutant LIGHT protein or a fragment thereof into an individual at a tumor site, wherein the mutant LIGHT protein or fragment does not have a proteolytic site; and
(b) reducing or controlling metastasis by inducing T-cell generation.
41 . The method of claim 40 , wherein the generated T-cells are activated within a tumor site.
42 . The method of claim 40 , wherein the generated T-cells circulate in blood.
43 . The method of claim 42 , wherein the circulating T-cells are cancer specific.
44 . The method of claim 40 , wherein the mutant LIGHT protein or the fragment is introduced into a tumor cell in vitro and the tumor cell expressing the mutant LIGHT protein or the fragment is delivered to the individual.
45 . The method of claim 40 , wherein the T-cell generation is CD8+ dependent.