Laser-scatter measurement instrument having carousel-based fluid sample arrangement
An instrument determines a concentration of bacteria in a plurality of fluid samples, and comprises a housing, a rotatable platform, a plurality of fluid containers, a light source, a sensor, and a motor. The rotatable platform is within the housing. The fluid containers are located on the rotatable platform. Each fluid container holds a corresponding one of the plurality of fluid samples, and has an input window and an output window. The light source provides an input beam for transmission into the input windows of the fluid containers and through the corresponding fluid samples. The input beam creates a forward-scatter signal associated with the concentration of bacteria. The motor rotates the rotatable platform so that the input beam sequentially passes through each fluid sample. A sensor within the housing detects the forward-scatter signal exiting from the output window associated with the fluid sample receiving the input beam.
1. An optical measuring instrument for determining a concentration of bacteria in a plurality of fluid samples, comprising:
a housing;
a rotatable platform within the housing;
a plurality of fluid containers coupled to the rotatable platform, each of the fluid containers holding a corresponding one of the plurality of fluid samples, each of the fluid containers having an input window and an output window;
a light source within the housing for providing an input beam for transmission into the input windows of the fluid containers and through the corresponding fluid samples, wherein the input window and the output window are vertically arranged with the fluid sample located therebetween,
a first mirror mounted to reflect the input beam in a vertical direction through the input window, the input beam creating a forward-scatter signal associated with the concentration of bacteria;
a motor for rotating the rotatable platform so that the input beam sequentially passes through each of the plurality of fluid samples; and
at least one sensor within the housing below the rotatable platform optically coupled to the light source in fixed orientation to the mirror, for detecting the forward-scatter signal exiting from the output window associated with the fluid sample receiving the input beam for analysis of bacteria in the fluid sample.
2. The optical measuring instrument of claim 1 , further including a heating system within the housing to maintain the fluid samples at a desired temperature to encourage bacterial growth in the fluid samples over a period of time.
3. The optical measuring instrument of claim 2 , wherein the heating system includes a plurality of heat lamps that provide energy to a bottom surface of the rotatable platform, a top surface of the rotatable platform receiving the plurality of fluid containers.
4. The optical measuring instrument of claim 1 , wherein the plurality of fluid containers are a part of a cuvette, the cuvette having an arc-shape and being registered on the rotatable platform.
5. The optical measuring instrument of claim 1 , wherein the rotatable platform has a plurality of openings that are aligned with the output windows of the cuvette.
6. The optical measuring instrument of claim 1 , wherein the plurality of fluid containers include at least one of (i) a single chemoeffector at different concentrations and (ii) different chemoeffectors, the plurality of fluid samples being derived from the same fluid sample source to determine the effects of the chemoeffector on the bacterial concentration of the respective fluid sample.
7. The optical measuring instrument of claim 1 , further comprising a second light source within the housing for providing a second input beam for transmission into the input windows of the fluid containers and though the corresponding fluid samples, the second input beam creating a forward-scatter signal associated with the concentration of bacteria, the light source for measuring a first group of the fluid samples and the second light source for measuring a second group of the fluid samples.
8. The optical measuring instrument of claim 7 , wherein the plurality of fluid containers are contained in a cuvette, the first group of the fluid samples being located in the cuvette on first locus have a first radius, the second group of the fluid samples being located in the cuvette on second locus have a second radius, the first radius being different from the second radius.
9. The optical measuring instrument of claim 1 , further comprising a door coupled to the housing, the door including a door platform that extends inwardly into the housing when the door is positioned in a closed state.
10. The optical measuring instrument of claim 1 , further comprising:
a second light source for providing a second input beam for transmission into the input windows of the fluid containers and through the corresponding fluid samples; and
a second mirror for turning the second input beam toward the input windows of the fluid containers.
11. The optical measuring instrument of claim 1 , wherein each of the plurality of fluid containers incudes a plurality of optical chambers and a plurality of fluid-input ports for filling the plurality of optical chambers, each of the fluid-input ports having an opening positioned, with respect to gravity, above the input and output windows for the respective optical chamber.
12. The optical measuring instrument of claim 11 , wherein each of the plurality of fluid containers includes a main body and a top cover, the input window for each of the plurality of optical chambers being located on the top cover, the plurality of optical chambers being located in the main body, the top cover being initially removed from the main body to permit the filling of the optical cavities with the plurality of fluid samples, the top cover being attachable to the main body after the plurality of fluid samples are filled within respective ones of the optical chambers.