Dry fractionation for plant based protein extraction
A dry fractionation method and system provides for generating a protein concentrate product therefrom. The method and system includes milling a plant-based flour to generate milled flour and generating a first protein concentrate from the milled flour using an air classifier. The method and system includes processing the first protein concentrate to generate a protein rich curd and generating a neutral hydrolyzed protein slurry by mixing the protein curd with a base, water and enzymatic cocktail. The method and system includes generating a homogenized protein slurry from the protein slurry and generating a cooled protein slurry by pasteurizing the homogenized protein slurry. Therein, the method and system provides for extracting the protein concentrate product from the cooled protein slurry.
1. A method for generating a protein concentrate product from a plant-based flour, the method comprising:
milling the plant-based flour to generate milled flour;
generating a first protein concentrate from the milled flour using a first air classifier;
generating a first starch concentrate from the air classifier;
recycling the first starch concentrate back to the first air classifier;
processing the first protein concentrate to generate a protein rich curd;
generating a neutral hydrolyzed protein slurry by mixing the protein rich curd with a base, an enzymatic cocktail and water;
generating a homogenized protein slurry from the neutral hydrolyzed protein slurry;
generating a cooled protein slurry by pasteurizing the homogenized protein slurry; and
extracting the protein concentrate product from the cooled protein slurry.
2. The method of claim 1 further comprising:
generating a second protein concentrate using the first starch concentrate with a second air classifier; and
generating the protein rich curd using the second protein concentrate.
3. The method of claim 2 further comprising:
generating a second starch concentrate using the second air classifier.
4. The method of claim 1 , wherein the milling the plant-based flour is performed using a jet mill.
5. The method of claim 1 , wherein generating the protein rich curd comprises:
mixing the first protein concentrate with water to generate a protein concentrate mixture;
separating the protein concentrate mixture to extract the protein rich curd therefrom.
6. The method of claim 5 further comprising:
additionally mixing the protein concentrate mixture with an enzyme mixture and an acid; and
separating sugar and water from the protein concentrate mixture.
7. The method of claim 5 further comprising:
additionally mixing the protein concentrate mixture with a base;
separating the protein concentrate mixture having the base therein, to extract a solubilized protein rich stream;
mixing the solubilized protein rich stream with an acid; and
extracting the protein curd from the solubilized protein rich stream having the acid mixed therewith.
8. The method of claim 5 further comprising:
additionally mixing the protein concentrate with a first acid;
separating the protein concentrate mixture having the acid therein, to extract an intermediate protein rich curd;
mixing the intermediate protein rich curd with a second acid; and
extracting the protein rich curd from the intermediate protein rich curd having the acid mixed therewith.
9. The method of claim 1 , wherein the plant-based flour is de-oiled prior to milling.
10. A system for generating a protein concentrate product from a plant-based flour, the system comprising:
a mill generating milled flour from the plant-based flour;
a first air classifier generating a first protein concentrate and a first starch concentrate from the milled flour, the first starch concentrate being recycled as an input to the first air classifier;
a processor generating a protein rich curd from the first protein concentrate;
a mixer generating a neutral hydrolyzed protein slurry from the protein curd, a base, an enzymatic cocktail and water;
a homogenizer generating a homogenized protein slurry from the neutral hydrolyzed protein slurry;
a pasteurizer generating a cooled protein slurry from the homogenized protein slurry; and
an extraction device for extracting the protein concentrate from the cooled protein slurry.
11. The system of claim 10 further comprising:
a second air classifier generating a second protein concentrate using the first starch concentrate; and
the processor generating the protein rich curd using the second protein concentrate.
12. The system of claim 10 , wherein the mill is a jet mill.
13. The system of claim 10 , wherein the processor further comprises:
a first mixer to mix the first protein concentrate with water to generate a protein concentrate mixture; and
a first separator to separate the protein concentrate mixture and extract the protein rich curd therefrom.
14. The system of claim 13 further comprising:
the first mixer further mixing the protein concentrate mixture with an enzyme mixture and an acid; and
the first separator separating sugar and water from the protein concentrate mixture.
15. The system of claim 13 further comprising:
the first mixer further mixing the protein concentrate mixture with a base;
the first separator separating the protein concentrate mixture having the base therein, to extract a solubilized protein rich stream;
a second mixer mixing the solubilized protein rich stream with an acid; and
a second separator extracting the protein curd from the solubilized protein rich stream having the acid mixed therewith.
16. The system of claim 13 further comprising:
the first mixer further mixing the protein concentrate with a first acid;
the first separator separating the protein concentrate mixture having the acid therein, to extract an intermediate protein rich curd;
a second mixer mixing the intermediate protein rich curd with a second acid; and
a second extractor extracting the protein rich curd from the intermediate protein rich curd having the acid mixed therewith.
17. The system of claim 10 , wherein the plant-based flour is de-oiled prior to milling.
18. A method for generating a protein concentrate product from a plant-based flour, the method comprising:
decorticating a plant-based source material, the source material having a protein and an oil content therein;
creating a flour from the source material having the cortex removed therefrom;
removing at least a portion of the oil content from the flour by generating an ethanol mixture by mixing the flour with ethanol;
extracting a de-oiled flour from the ethanol mixture;
milling the de-oiled flour to generate milled flour;
generating a first protein concentrate from the milled flour using a first air classifier;
processing the first protein concentrate to generate a protein rich curd;
generating a neutral hydrolyzed protein slurry by mixing the protein rich curd with a base, an enzymatic cocktail and water;
generating a homogenized protein slurry from the neutral hydrolyzed protein slurry;
generating a cooled protein slurry by pasteurizing the homogenized protein slurry; and
extracting the protein concentrate product from the cooled protein slurry.
19. A system for generating a protein concentrate product from a plant-based flour, the system comprising:
a decortication device removing cortex from plant-based source material, the source material having a protein and an oil content therein;
a milling device milling the source material having the cortex removed therefrom to create a flour;
an oil removal device removing at least a portion of the oil content from the flour by mixing the flour with ethanol to form an ethanol mixture and extracting a de-oiled flour
a mill generating milled flour from the flour;
a first air classifier generating a first protein concentrate from the milled flour;
a processor generating a protein rich curd from the first protein concentrate;
a mixer generating a neutral hydrolyzed protein slurry from the protein rich curd, a base, an enzymatic cocktail and water;
a homogenizer generating a homogenized protein slurry from the neutral hydrolyzed protein slurry;
a pasteurizer generating a cooled protein slurry from the homogenized protein slurry; and
an extraction device for extracting the protein concentrate from the cooled protein slurry.