Porous structure and method for manufacturing same
A porous structure according to the present invention has a polymerase chain reaction (PCT) primer inside pores thereof, and hence, even an inner portion thereof can be used unlike general structures of which only surfaces are used for amplification and detection, thereby maximizing reactivity. In addition, the differentiating of the kinds of primers contained in respective structures leads to detection of several kinds of target nucleic acids at the same and real-time analysis thereof at the same time, and thus is useful for multiplex real-time PCR.
1. A porous structure comprising a cured polymer having pores,
the porous structure comprising primers in one or more directions between a forward primer and a reverse primer of a target nucleic acid as a primer of polymerase chain reaction (PCR) fixed to the interior of the pores,
wherein a porosity of the porous structure is from 60 vol % to 80 vol % with respect to a total volume of the porous structure.
2. The porous structure according to claim 1 , wherein a primer only in one direction between a forward primer and a reverse primer of a target nucleic acid is fixed to the porous structure as a primer of polymerase chain reaction (PCR).
3. The porous structure according to claim 1 , wherein the porous structure is from 10 μm to 500 μm in size.
4. The porous structure according to claim 1 , wherein a primer fixed to the interior of a pore of the porous structure includes an acryl group at a terminal, wherein the acryl group is chemically fixed to the porous structure.
5. The porous structure according to claim 1 , wherein one or more primers among the primers fixed to the interior of a pore of the porous structure are linked to the porous structure via a linker.
6. The porous structure according to claim 5 , wherein a length of the linker is from 5 nm to 100 nm.
7. The porous structure according to claim 1 , further comprising an encoder to provide information corresponding to a fixed primer.
8. An apparatus for multiplex real-time nucleic acid amplification comprising:
one or more porous structures according to claim 1 ; and
an array having a surface patterned in a well form so that the porous structure is arranged.
9. The apparatus for multiplex real-time nucleic acid amplification according to claim 8 , comprising a plurality of porous structures including a primer for each of different target nucleic acids.
10. The apparatus for multiplex real-time nucleic acid amplification according to claim 8 , wherein a plurality of the one or more porous structures have different sizes depending on the kind of primers included therein.
11. The porous structure according to claim 1 , further comprising a fluorescent marker to provide quantitative information corresponding to a nucleic acid to be amplified in a pore.
12. The porous structure according to claim 1 , wherein the size of the porous structure is configured to identify the primer of PCR fixed to the interior of the pores.
13. The porous structure according to claim 1 , wherein the primer is fixed to the interior of the pores in a concentration of from 10 μM to 100 μM.