Porous particle composite for PCR with heat dissipation function
The present invention relates to a porous particle composite for PCR, wherein the porous particle composite distributes photothermal nano-elements that generate heat by absorbing light in porous particles in which nucleic acid amplification occurs through temperature control so as not to adjust the temperature of the entire sample by using a hot plate or the like but to adjust the temperature inside the particles by irradiating light to the porous particles to allow nucleic acid amplification inside thereof, thereby reducing energy consumption and shortening diagnostic time.
1. A porous particle composite for PCR, comprising porous particles and photothermal nanoelements;
wherein the porous particles are a hydrophilic polymer in solid state,
wherein a porosity of the porous particles is 10-95 v/v % and a particle diameter of the porous particles is 10 μm to 1 mm;
wherein the photothermal nanoelements have a diameter or length of 1-500 nm and are fixed to the porous particles;
wherein when exposed to light having a wavelength of 800 nm the photothermal nanoelements absorb the light and generate heat; and
wherein the photothermal nanoelements are not rod-shaped.
2. The porous particle composite for PCR according to claim 1 , wherein the photothermal nanoelement is any one or more selected from a group consisting of a metal nanoparticle, a carbon nanoelement, an inorganic nanoelement, an organic nanoelement, a quantum dot and a rare-earth element;
wherein the carbon nanoelement is not a carbon nanotube.
3. The porous particle composite for PCR according to claim 2 , wherein 0.25-50 mg of the photothermal nanoelements are present in 1 mL of the porous particles.
4. The porous particle composite for PCR according to claim 3 , wherein the photothermal nanoelements are physically or chemically fixed to the porous particles.
5. The porous particle composite for PCR according to claim 4 , wherein one primer of a forward primer and a reverse primer is fixed inside the porous particles, the other primer is physically or chemically attached to the surface of the photothermal nanoelements fixed inside the porous particles, and the other primer is detached from the photothermal nanoelements due to heat generated in the photothermal nanoelements when light is irradiated to the porous particle composite for PCR.
6. A method for preparing the porous particle composite for PCR according to claim 1 , the method comprising: a binding step of, by mixing one primer of a forward primer and a reverse primer in a solution wherein photothermal nanoelements are dispersed, binding the one primer onto the surface of the photothermal nanoelements; a first mixing step of forming a first mixture solution by mixing the photothermal nanoelements with the one primer bound and a porous particle-forming polymer solution; a second mixing step of forming a second mixture solution by mixing the first mixture solution with the other primer; and a curing step of preparing a porous particle composite having porosity, having the other primer of target nucleic acids fixed to inner pores and having the photothermal nanoelements with the one primer attached fixed to the inner pores by curing the second mixture solution.
7. The method for preparing a porous particle composite for PCR according to claim 6 , wherein the binding step is performed by mixing one primer of a forward primer and a reverse primer in a solution wherein photothermal nanoelements are dispersed and conducting sonication for 30-60 minutes.
8. A nucleic acid amplification device comprising a PCR chip comprising a porous particle composite which amplifies target nucleic acids by polymerase chain reaction inside thereof, a light irradiation unit which irradiates light to the porous particle composite and a controller which adjusts the light irradiated by the light irradiation unit such that the porous particle composite has a temperature where polymerase chain reaction occurs, wherein the porous particle composite is the porous particle composite according to claim 1 .
9. The nucleic acid amplification device according to claim 8 , wherein the intensity of the light irradiated by the light irradiation unit is controlled by controlling any one or more of the current, voltage, duty cycle and frequency of power applied to the light irradiation unit.
10. The nucleic acid amplification device according to claim 8 , wherein the controller comprises a light setting unit which sets the condition of the light irradiated by the light irradiation unit such that the porous particle composite has a temperature where polymerase chain reaction occurs.
11. The nucleic acid amplification device according to claim 8 , wherein the nucleic acid amplification device further comprises a measurement unit which measures the porous particle composite located in the PCR chip to which light has been irradiated by the light irradiation unit and the controller comprises a result analysis unit which determines the presence or amount of target nucleic acids by analyzing the result measured by the measurement unit.
12. A nucleic acid amplification method comprising a sample reaction step of reacting a porous particle composite which amplifies target nucleic acids by polymerase chain reaction inside thereof with a sample, a light irradiation step of irradiating light to the porous particle composite which has reacted with the sample such that the porous particle composite has a temperature where polymerase chain reaction occurs after the sample reaction step, a measurement step of measuring the change of the porous particle composite after the light irradiation step and an analysis step of determining the presence or amount of target nucleic acids by analyzing the result measured in the measurement step, wherein the porous particle composite is the porous particle composite according to claim 1 .
13. The porous composite for PCR according to claim 5 wherein the other primer is chemically attached to the surface of the photothermal nanoelements by a linker having a length of 1-100 nm.