Genetically encoded fluorescent sensors for detecting ligand bias and intracellular signaling through cAMP pathways
Described herein are novel fluorescent sensors for cyclic adenosine monophosphate (cAMP) that are based on single fluorescent proteins. These sensors use less visible spectrum than FRET-based sensors, produce robust changes in fluorescence, and can be combined with one another, or with other sensors, in a multiplex assay on standard fluorescent plate readers or live cell imaging systems.
1. A nucleic acid molecule encoding a cyclic adenosine monophosphate (cAMP) sensor protein comprising a first polypeptide linked to a second polypeptide, wherein the first polypeptide comprises an amino acid sequence at least 95% identical to amino acids 1-292 of SEQ ID NO: 6; and
the second polypeptide comprises a circularly permuted, fluorescent protein, wherein the fluorescent protein is selected from the group consisting of GFP, eGFP, eYFP, Emerald, mApple, mPlum, mCherry, tdTomato, mStrawberry, J-Red, DsRed-monomer, mOrange, MKO, mCitrine, Venus, YPet, CyPet, mCFPm, Cerulean, T-Sapphire, mKOK, mUKG, Clover, mKate, tagRFP, tagGFP, and mNEON green,
wherein binding of cAMP to the first polypeptide alters the level of fluorescence from the second polypeptide, wherein the C-terminal amino acid of the amino acid sequence at least 95% identical to amino acids 1-292 of SEQ ID NO: 6 is covalently bound to a linker of 1-10 amino acids, and wherein the C-terminal amino acid of the linker is covalently bound to the N-terminal amino acid of the circularly permuted, fluorescent protein.
2. A method of detecting changes in the intracellular level of cAMP, comprising:
a. introducing into a cell a nucleic acid molecule encoding a cAMP sensor protein comprising a first polypeptide linked to a second polypeptide, wherein the first polypeptide comprises an amino acid sequence at least 95% identical to amino acids 1-292 of SEQ ID NO: 6; and the second polypeptide comprises a circularly permuted, fluorescent protein, wherein the fluorescent protein is selected from the group consisting of GFP, eGFP, eYFP, Emerald, mApple, mPlum, mCherry, tdTomato, mStrawberry, J-Red, DsRed-monomer, mOrange, MKO, mCitrine, Venus, YPet, CyPet, mCFPm, Cerulean, T-Sapphire, mKOK, mUKG, Clover, mKate, tagRFP, tagGFP, and mNEON green,
wherein binding of cAMP to the first polypeptide alters the level of fluorescence from the second polypeptide, wherein the C-terminal amino acid of the amino acid sequence at least 95% identical to amino acids 1-292 of SEQ ID NO: 6 is covalently bound to a linker of 1-10 amino acids, and wherein the C-terminal amino acid of the linker is covalently bound to the N-terminal amino acid of the circularly permuted, fluorescent protein; and
b. detecting changes in the intracellular level of fluorescence produced by the fluorescent protein.
3. The nucleic acid molecule of claim 1 , wherein the first polypeptide comprises an amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO:37.
4. The method of claim 2 , wherein the first polypeptide comprises an amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO:37.
5. The nucleic acid molecule of claim 1 , wherein the first polypeptide comprises an amino acid sequence at least 99% identical to amino acids 1-292 of SEQ ID NO: 6.
6. The nucleic acid molecule of claim 1 , wherein the first polypeptide comprises amino acids 1-292 of SEQ ID NO: 6.
7. The nucleic acid molecule of claim 1 , wherein the second polypeptide comprises mNEON green.
8. The nucleic acid molecule of claim 5 , wherein the second polypeptide comprises mNEON green.
9. The method of claim 2 , wherein the first polypeptide comprises an amino acid sequence at least 99% identical to amino acids 1-292 of SEQ ID NO: 6.
10. The method of claim 2 , wherein the first polypeptide comprises amino acids 1-292 of SEQ ID NO: 6.
11. The method of claim 2 , wherein the second polypeptide comprises mNEON green.
12. The method of claim 9 , wherein the second polypeptide comprises mNEON green.