Compositions and methods for delivery of bioencapsulated proteins across blood-brain and retinal barriers
Compositions and methods effective to provide therapeutic benefit for disorders of the central nervous system via oral or systemic delivery of therapeutic proteins produced in plastids are disclosed.
1. A method for delivery of human myelin basic protein (MBP) across the blood brain barrier and blood retinal barrier in a subject in need thereof, said subject having a central nervous system disorder, comprising:
a) administering to the subject a composition comprising lyophilized leaf material containing an effective amount of a fusion protein comprising said MBP operably linked to cholera toxin B (CTB) subunit in a 1:1 ratio under conditions whereby said fusion protein crosses the blood brain and blood retinal barrier and wherein said composition is administered orally and effective to
i) reduce levels of amyloid beta protein in the brain; and/or
ii) reduce amyloid levels in the retina; and/or
iii) prevent loss of retinal ganglion cells; and/or
iv) decrease the ratio of insoluble vs soluble amyloid beta protein42 in hippocampus;
said CTB-MBP fusion protein being present in a plastid transformed with a stable plastid transformation vector and expression vector which expresses said CTB-MBP fusion protein in said leaf material, wherein said CTB and human MBP are separated by a glycine-proline-glycine-proline hinge region and a furin site,
wherein the subject is assessed for the reduction of amyloid plaque levels.
2. The method of claim 1 , wherein said subject is a human or nonhuman mammal.