Apparatus and methods for aspirating and separating components of different densities from a physiological fluid containing cells
A system for separating components defining a cavity for receiving the fluid. The system further includes a rigid insert slidably disposed in the cavity, the insert including a funnel-shaped upper portion and a hole therethrough. The insert has a density such that upon centrifugation a selected component of the fluid resides within the upper portion of the insert. A bone marrow aspiration device includes an introducer needle assembly and an aspiration needle assembly. The introducer needle assembly includes an introducer cannula and a removable trocar. The aspiration needle assembly includes a flexible aspiration cannula and a flexible stylet. The length of the aspiration cannula is substantially greater than the length of the introducer cannula. The aspiration needle assembly is receivable in the introducer cannula when the trocar is removed from the introducer needle assembly. The aspiration cannula forms a channel for aspirating bone marrow when the stylet is removed.
1. A system for separating components of different densities from a physiological fluid containing cells using a centrifuge, the system comprising:
a container, having a bottom, a top disposed opposite the bottom, and a sidewall extending between the bottom and the top, the container defining a cavity for receiving the fluid;
a rigid insert slidably disposed in the cavity, the insert having a density such that upon centrifugation a selected component of the fluid resides within an upper portion of the insert;
a container port disposed in the top of the container;
a cannula assembly including a cannula receivable in the container port to extend into the upper portion and withdraw the selected component from the upper portion of the insert through a lower port in the cannula, the cannula including an upper port displaced from a distal end of the cannula to withdraw fluid at a predetermined height above the distal end of the cannula; and
a clamping mechanism to hold the insert in place after centrifugation, wherein the clamping mechanism is coupled to the container sidewall to press the sidewall inward against the insert.
2. The system of claim 1 , wherein the insert has a hole therethrough, and wherein the distal end of the cannula is a closed end to close the hole in the insert.
3. The system of claim 2 , wherein the cannula assembly includes an inner cannula coaxially disposed within an outer cannula, and wherein the lower port is formed by a first set of side ports into the cannulae to open a first channel through the cannula assembly for withdrawing the selected component from the upper portion of the insert with alignment of the first set of ports with relative rotation of the inner and outer cannulae.
4. The system of claim 3 , wherein the upper port is formed by a second set of side ports into the cannulae to open a second channel through the cannula assembly for withdrawing a component other than the selected component with alignment of the second set of ports with relative rotation of the inner and outer cannulae.
5. The system of claim 4 , wherein alignment of the first set of ports causes misalignment of the second set of ports, thereby closing the second channel, and alignment of the second set of ports causes misalignment of the first set of ports, thereby closing the first channel.
6. The system of claim 4 , wherein the selected component is buffy coat and the component other than the selected component is blood plasma.
7. The system of claim 1 , wherein volume contained in the upper portion is between 5% and 20% of the volume of the container cavity.