Reversible covalent linkage of functional molecules
The present invention relates to the use of a compound containing a moiety of formula (I) as a reagent for linking a compound of formula R 1 —H which comprises a first functional moiety of formula F 1 to a second functional moiety of formula F 2 wherein X, X′, Y, R 1 , F 1 and F 2 are as defined herein. The present invention also provides related processes and products. The present invention is useful for creating functional conjugate compounds, and specifically conjugates in which at least one of the constituent molecules carries a thiol group.
1. A compound of formula (IIIa)
wherein:
R 1 is an antibody or antibody fragment that is capable of binding to a specific antigen via an epitope on the antigen, said antibody or antibody fragment containing a first cysteine residue and a second cysteine residue;
R 1 is attached at the 2-position in the compound of formula (IIIa) by a thiol bond —S— which corresponds to the thiol group in said first cysteine residue;
R 1 is attached at the 3-position in the compound of formula (IIIa) by a thiol bond —S— which corresponds to the thiol group in said second cysteine residue;
F 2 is a drug; and
L represents a linker group.
2. A compound according to claim 1 , wherein the first cysteine residue of R 1 and the second cysteine residue of R 1 in the compound of formula (IIIa) are derived from an internal disulfide bridge formed in the antibody or antibody fragment.
3. A compound according to claim 1 , wherein R 1 is an antibody.
4. A compound according to claim 1 , wherein the drug is a cytotoxic agent.
5. A compound according to claim 3 , wherein F 2 is a cytotoxic agent.