Human antibody molecules for IL-13
Specific binding members, in particular human anti-IL-13 antibody molecules and especially those which neutralize IL-13 activity. Methods for using anti-IL-13 antibody molecules in diagnosis or treatment of IL-13 related disorders, including asthma, atopic dermatitis, allergic rhinitis, fibrosis, inflammatory bowel disease and Hodgkin's lymphoma.
1. An isolated nucleic acid molecule comprising a polynucleotide encoding an antibody or antigen-binding fragment thereof that binds human interleukin-13 (IL-13), wherein the antibody or antigen-binding fragment thereof comprises a set of complementarity determining regions (CDRs) HCDR1, HCDR2, HCDR3, LCDR1, LCDR2 and LCDR3, wherein:
HCDR1 comprises SEQ ID NO:7;
HCDR2 comprises SEQ ID NO:8;
HCDR3 comprises SEQ ID NO:9;
LCDR1 comprises SEQ ID NO:10;
LCDR2 comprises SEQ ID NO:11; and
LCDR3 comprises SEQ ID NO:12.
2. The nucleic acid molecule according to claim 1 , wherein the nucleic acid molecule is operably linked to a control sequence.
3. A vector comprising the nucleic acid molecule according to claim 2 .
4. A host cell transformed with the vector of claim 3 .
5. The host cell of claim 4 , wherein the host cell is a mammalian host cell.
6. The mammalian host cell of claim 5 , wherein the host cell is a Chinese hamster ovary (CHO) cell, a HeLa cell, a baby hamster kidney cell, a NS0 mouse melanoma cell, a YB2/0 rat myeloma cell, a human embryonic kidney cell, or a human embryonic retina cell.
7. A method of making an antibody or antigen-binding fragment thereof that binds human interleukin-13 (IL-13) comprising culturing a host cell according to claim 4 under suitable conditions for producing the antibody or antigen-binding fragment thereof.
8. The method of claim 7 further comprising isolating the antibody or antigen-binding fragment thereof secreted from the host cell.
9. An isolated nucleic acid molecule comprising a polynucleotide encoding an antibody or antigen-binding fragment thereof that binds human interleukin-13 (IL-13), wherein the antibody or antigen-binding fragment thereof comprises the BAK502G9 heavy chain variable region (SEQ ID NO: 15) and the BAK502G9 light chain variable region (VL) (SEQ ID NO:16).
10. The nucleic acid molecule according to claim 9 , wherein the nucleic acid molecule is operably linked to a control sequence.
11. A vector comprising the nucleic acid molecule according to claim 10 .
12. A host cell transformed with the vector of claim 11 .
13. The host cell of claim 12 , wherein the host cell is a mammalian host cell.
14. The mammalian host cell of claim 13 , wherein the host cell is a Chinese hamster ovary (CHO) cell, a HeLa cell, a baby hamster kidney cell, a NS0 mouse melanoma cell, a YB2/0 rat myeloma cell, a human embryonic kidney cell, or a human embryonic retina cell.
15. A method of making an antibody or antigen-binding fragment thereof that binds human interleukin-13 (IL-13) comprising culturing a host cell according to claim 12 under suitable conditions for producing the antibody or antigen-binding fragment thereof.
16. The method of claim 15 further comprising isolating the antibody or antigen-binding fragment thereof secreted from the host cell.
17. An isolated nucleic acid molecule comprising SEQ ID NO:111 or SEQ ID NO:112.
18. A host cell transformed with the nucleic acid molecule of 17 .
19. The host cell of claim 18 , wherein the host cell is a Chinese hamster ovary (CHO) cell, a HeLa cell, a baby hamster kidney cell, a NS0 mouse melanoma cell, a YB2/0 rat myeloma cell, a human embryonic kidney cell, or a human embryonic retina cell.