Composition and method for delivery of living cells in a dry mode having a surface layer

1. A method of transporting living cells from a first location to a second location, the method comprising:

providing living cells, a first mixture, and an inert carrier substrate at a first location, wherein the first mixture comprises nutrients and a carrier fluid, and wherein the inert carrier substrate comprises preformed particles;

mixing the living cells with the first mixture to form a second mixture at the first location;

mixing the second mixture with the inert carrier substrate to form a loaded product at the first location, wherein the loaded product is a granular composition and is substantially dry to the touch; and

transporting the loaded product to a second location without significant degradation of the living cells.

2. The method as claimed in claim 1 , further comprising the step of contacting the loaded product with a releasing solution to release the living cells from the inert carrier substrate at the second location.

3. The method as claimed in claim 2 , wherein the releasing solution comprises water or saline.

4. The method as claimed in claim 2 , further comprising isolating the living cells from the releasing solution.

5. The method as claimed in claim 1 , wherein the second mixture contains at least about 3% living cells by volume.

6. The method as claimed in claim 1 , wherein the second mixture contains about 5% living cells by volume.

7. The method as claimed in claim 1 , wherein the loaded product is transported without the use of a refrigerant.

8. The method as claimed in claim 7 , wherein the loaded product is transported in a non-frozen state.

9. The method as claimed in claim 1 , wherein the loaded product is stored from about 33° F. to about 80° F.

10. The method as claimed in claim 1 , wherein the second mixture contains from about 1% to about 30% living cells by volume.

11. The method as claimed in claim 1 , wherein the performed particles comprise pores, and wherein the pores have diameters from about 38 nanometers to about 240 nanometers.

12. The method as claimed in claim 1 , wherein the performed particles have diameters from about 10 micrometers to about 1400 micrometers.

13. The method as claimed in claim 1 , wherein the inert carrier substrate comprises precipitated silica, diatomaceous earth, walnut and pecan shells, rice hulls, cellulosic clay, montmorillonite clay, bentonite clay, wool, cotton, cellulose, corn cobs, cellulose shells, or any combination thereof.

14. The method as claimed in claim 1 , wherein the loaded product further comprises a surface layer.

15. The method as claimed in claim 14 , wherein the surface layer is permeable to carbon dioxide and oxygen.

16. The method as claimed in claim 1 , wherein the loaded product comprises an initial living cell count and wherein the loaded product maintains a living cell count that is from about 75% to about 400% of the initial living cell count after about 45 days.

17. The method as claimed in claim 1 , wherein the loaded product is formed without the use of a reaction.

18. The method as claim 1 , wherein the loaded product readily flows.

19. A method of transporting living cells from a first location to a second location, the method comprising:

preparing a granular composition at a first location, the composition comprising:

an inert carrier substrate comprising preformed particles, wherein the preformed particles comprise pores, and wherein living cells are contained within the pores; and

a surface layer disposed on an outer surface of the inert carrier substrate, wherein the surface layer is permeable to molecules that aid in cell growth of the living cells such that the composition is operable to allow for increased propagation of the living cells within the inert carrier substrate as compared to another composition having an absence of the surface layer; and

transporting the loaded product to a second location without significant degradation of the living cells.