Method and carrier complexes for delivering molecules to cells
The invention relates to carrier complexes and methods for delivering molecules to cells. The carrier complexes comprises a molecule and an aromatic cationic peptide in accordance with the invention. In one embodiment, the method for delivering a molecule to a cell comprises contacting the cell with a carrier complex. In another embodiment, the method for delivering a molecule to a cell comprises contacting the cell with a molecule and an aromatic cationic peptide.
1. A carrier complex comprising a molecule conjugated to an aromatic cationic peptide, wherein the aromatic cationic peptide is selected from the group consisting of:
Phe-D-Arg-Phe-Lys-NH 2 (Phe 1 -DALDA),
D-Arg-2′,6′Dmt-Lys-Phe-NH 2 , and
2′,6′-Dmp-D-Arg-Phe-Lys-NH 2 (Dmp 1 -DALDA),
and wherein the molecule is a lipid, an enzyme, an antibody, or a neurotrophic growth factor.
2. The carrier complex according to claim 1 , wherein the molecule is conjugated to the aromatic cationic peptide by a linker.
3. The carrier complex according to claim 2 , wherein the linker is a component of the aromatic-cationic peptide.
4. The carrier complex according to claim 2 , wherein the linker is a component of the molecule.
5. The carrier complex according to claim 1 , wherein the molecule and aromatic cationic peptide are chemically bonded.
6. The carrier complex according to claim 1 , wherein the molecule and aromatic cationic peptide are physically bonded.
7. A method for delivering a molecule to a cell, the method comprising contacting the cell with a carrier complex, wherein the carrier complex comprises the molecule conjugated to an aromatic cationic peptide, wherein the aromatic cationic peptide is selected from the group consisting of:
Phe-D-Arg-Phe-Lys-NH 2 (Phe 1 -DALDA),
D-Arg-2′,6′-Dmt-Lys-Phe-NH 2 , and
2′,6′-Dmp-D-Arg-Phe-Lys-NH 2 (Dmp 1 -DALDA);
and wherein the molecule is a lipid, an enzyme, an antibody, or a neurotrophic growth factor.
8. The method according to claim 7 , wherein the molecule is conjugated to the aromatic cationic peptide by a linker.
9. The method according to claim 8 , wherein the linker is a component of the aromatic-cationic peptide.
10. The method according to claim 8 , wherein the linker is a component of the molecule.
11. The method according to claim 7 , wherein the molecule and aromatic cationic peptide are chemically bonded.
12. The method according to claim 7 , wherein the molecule and aromatic cationic peptide are physically bonded.