Method for diagnosing fibromyalgia syndrome, and kit therefor
The present invention provides a novel marker for diagnosing fibromyalgia syndrome (FMS). More specifically, the present invention provides a method for diagnosing FMS, comprising measurement of (1) the relative frequency of MAITs to the total T cells in a sample; or (2) the expression level of one or more surface antigens selected from the group consisting of CD4, CD8, CCR4, CCR7, CXCR1, NKp80, CD150, CD107a, CD8β, CD44 and CXCR4 for MAITs in a sample, wherein the sample is a biological sample collected from a human; and a diagnosis kit for FMS, comprising a means for measuring (1) the relative frequency of MAITs to the total T cells in a sample; or (2) the expression level of one or more surface antigens selected from the group consisting of CD4, CD8, CCR4, CCR7, CXCR1, NKp80, CD150, CD107a, CD8β, CD44 and CXCR4 for MAITs in a sample, wherein the sample is a biological sample collected from a human.
1. A method for detecting levels of mucosal associated invariant T cells (MAITs) or one or more surface antigens on MAITs, the method comprising
measuring (1) a frequency of MAITs relative to the total T cells in a sample, or (2) the expression level of one or more surface antigens on MAITs selected from the group consisting of CD4, CD8, CCR4, CCR7, CXCR1, NKp80, CD150, CD107a, CD8β, CD44 and CXCR4 in a sample, wherein said sample is a biological sample collected from a human having fibromyalgia syndrome or suspected of having fibromyalgia syndrome.
2. The method according to claim 1 , wherein said surface antigens are selected from the group consisting of CD4, CCR4, CCR7, CSCR1, NKp80, CD150, CD107a and CD8β.
3. The method according to claim 1 , wherein the expression level of one or more surface antigens selected from the group consisting of CCR7, NKp80, CD150 and CD8β on CD8 positive (CD8 − ) cells in said MAITs is measured.
4. The method according to claim 1 , wherein the expression level of one or more surface antigens selected from the group consisting of CCR4, CXCR1 and CD107a on CD4 and CD8 double negative (CD4 − CD8 − ) cells in said MAITs is measured.
5. The method according to claim 1 , wherein said surface antigen is CD44 or CXCR1 or both CD44 and CXCR1.
6. The method according to claim 1 , wherein the expression level of CXCR1 on the CD4 − CD8 − cells in said MAITs is measured.
7. The method according to claim 1 , wherein the surface antigen is CXCR4.
8. The method according to claim 1 , wherein the sample is peripheral blood.
9. The method according to claim 1 , wherein the expression level is a protein amount.
10. The method according to claim 9 , wherein the protein amount is measured with an antibody against the one or more surface antigens.
11. The method of claim 1 , comprising detecting the expression level of CD150 and at least one of (A) CD44, CXCR1, or both; (B) CRP, MMP-3, PVAS, FVAS, or a combination thereof.
12. A method for treating fibromyalgia syndrome (FMS) in a subject in need thereof, the method comprising administering an effective amount of a drug to the subject to treat FMS, wherein said drug is selected from the group consisting of methotrexate, sulfasalazine, a corticosteroid, an anticonvulsant, an anti-depressant, an opiate and neurotrophin, wherein the subject has been identified as a subject in need thereof by diagnosing FMS by using measured value(s) of surface expression level of CD150 on mucosal associated invariant T cells (MAITs) in a sample, and (1) a frequency of MAITs relative to the total T cells in a sample, or (2) the expression level of one or more surface antigens on MAITs selected from the group consisting of CD4, CD8, CCR4, CCR7, CXCRI, NKp80, CD107a, CD83, CD44 and CXCR4 in a sample, wherein said sample is a biological sample collected from a human.
13. The method of claim 12 , wherein the diagnosing further comprises differentiating an FMS subject from a healthy subject.