Bio-sensor circuit
A bio-sensor circuit for detecting characteristics of a substance placed thereupon, the bio-sensor circuit has: a printed circuit having at least one electrically conductive contact sensor on a surface of the printed circuit, the electrically conductive contact sensor including a biochemical agent; and an electrically insulative coating affixed to the surface of the printed circuit, the electrically insulative coating having a well associated with the electrically conductive contact sensor, thereby leaving the electrically conductive contact sensor exposed through the electrically insulative coating, the electrically insulative coating being a resinous compound formed by injection molding. An electrochemical change resulting from the interaction of the substance to be characterized and the biochemical agent creates a detectable electrical potential at the electrically conductive contact sensor. A system and method of creating such a circuit via injection molding is also provided.
1. A bio-sensor circuit system for the production of bio-sensor circuits comprising:
a bio-sensor production reel having an elongate substrate formed from a strip of electrically insulative material, the substrate including a plurality of index holes positioned symmetrically at or adjacent to both longitudinal sides of the substrate to enable actuation of the reel in a longitudinal direction, and a plurality of printed circuits printed in a parallel arrangement along the length of the substrate, the printed circuits each including at least one electrically conductive contact sensor;
an injection molding tool having upper and lower injection mold elements being disposed above and below upper and lower surfaces of the reel, the upper and lower injection mold elements being capable of being actuated into engagement with the reel, thereby forming a cavity about each printed circuit between the upper and lower injection mold elements, the injection molding tool further including at least one abutment element capable of engaging with a portion of the surface of the reel inside the cavity when the upper and lower injection mold elements are actuated into engagement with the reel; and
a sprocket feed roller capable of engagement with the index holes of the reel, wherein the roller is rotatable so as to effect actuation of the reel through the injection molding tool and to be spooled onwards and transferred in a reel-to-reel transferring.
2. A method of producing bio-sensor circuits using the bio-sensor circuit system of claim 1 , comprising the steps of:
a] actuating the reel using the sprocket feed roller so as to position at least one printed circuit between the upper and lower injection mold elements;
b] actuating the upper and lower injection mold elements towards one another so as to form the cavity about each printed circuit, such that the said at least one abutment element contacts a relevant portion of a surface of the said at least one electrically conductive contact sensor;
c] injecting a resinous substance into the cavity so as to cover an exposed area of a surface of the printed circuit;
d] allowing the resinous substance to set and affix to the surface of the printed circuit; and
e] separating the upper and lower injection mold elements leaving the set resinous substance as an electrically insulative coating on the surface of the printed circuit, a well being formed in the resinous substance at or adjacent the said at least one electrically conductive contact sensor as a result of the positioning of each abutment element, thereby forming the bio-sensor circuit;
f] actuating the reel using the sprocket feed roller so as to position another at least one printed circuit between the upper and lower injection mold elements and enable the reel to be spooled onwards to achieve a repeatable reel-to-reel process.
3. The method of claim 2 , further comprising step g], subsequent to step f], of separating the individually formed bio-sensor circuits from the reel following formation to form discrete bio-sensor circuits.
4. The method of claim 2 , wherein in step d], the resinous substance set and affixed to the surface of the printed circuit has a depth between 50 and 300 μm to keep the reel to be woundable.