Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting
In certain embodiments this invention provides a pulsed-laser triggered microfluidic switching mechanism that can achieve a switching time of 70 μs. This switching speed is two orders of magnitude shorter than that of the fastest switching mechanism utilized in previous μFACS.
1. A high-speed microfluidic switch, said switch comprising:
a first microfluidic channel comprising a split into a plurality of paths, said plurality of paths comprising at least a first path and a second path;
a liquid or gel filled second channel adjacent to said first microfluidic channel where said liquid or gel flows through said second channel and said second channel is disposed such that formation of a gas or plasma bubble in said second channel redirects particles flowing into said first path so they flow into said second path; and
a pulse laser focused on said second channel and configured to provide sufficient energy to form said gas or plasma bubble;
wherein said switch comprises a wall between said second channel and said first microfluidic channel where said wall is disposed so that formation of a gas or plasma bubble in said second channel deforms said wall to redirect particles flowing into said first path so they flow into said second path.
2. The switch of claim 1 , wherein said first microfluidic channel is a Y-shaped microchannel.
3. The switch of claim 1 , wherein said first microfluidic channel is formed from an elastomeric material.
4. The switch of claim 3 , wherein said elastomeric material is PDMS.
5. The switch of claim 1 , wherein said pulse laser is configured to operate at a frequency that provides a switching time for said switch of less than about 100 μsec.
6. The switch of claim 1 , wherein said second channel contains a liquid.
7. The switch of claim 1 , wherein said liquid or gel comprises light-absorbing nanoparticles and/or microparticles.
8. The switch of claim 1 , wherein said switch is disposed on a substrate comprising a material selected from the group consisting of a polymer, a plastic, a glass, quartz, a dielectric material, a semiconductor, silicon, germanium, ceramic, and a metal or metal alloy.
9. The switch of claim 1 , wherein said switch is integrated with other microfluidic components selected from the group consisting of PDMS channels, wells, and valves.
10. The switch of claim 1 , wherein said switch is a component of a lab-on-a-chip.
11. The switch of claim 1 , wherein said switch is a component of a cell sorter.
12. A system for controlling microfluidic flow, said system comprising a switch according to claim 1 , and a controller that controls said laser.
13. The system of claim 12 , wherein said controller controls the pulse energy produced by said laser and the induced bubble size.
14. The system of claim 12 , wherein said system further comprises components for detecting particles or cells in said system.
15. A method for detecting or sorting particles or cells, said method comprising flowing said particles or cells through a switch according to claim 1 , and activating said switch to channel desired particles into a desired flow path.