Pressure transmission liquid for cellular analyzer, cellular analyzer and method for analyzing a liquid cellular sample
A pressure transmission liquid for a cellular analyzer, a system for transferring a liquid cellular sample for analysis by a cellular analyzer, and a method for transferring a liquid cellar sample for analysis are disclosed. The pressure transmission liquid includes an aqueous solution which is isotonic and substantially non-conductive characteristics. The cellular analyzer includes a pipetting module having a pipetting tip, a device for positioning the pipetting module, a sensor for detecting a liquid level of a liquid cellular sample to be analyzed, a pressure transmission liquid, and a pressure transmission liquid conduit connected to the pipetting tip and the pressure transmission liquid reservoir.
1. A system for transferring a liquid cellular sample for analysis by a cellular analyzer comprising:
a pipetting module having a pipetting tip, the pipetting module comprising a fluid sample conduit configured for receiving fluid samples aspirated through the pipetting tip;
an automated positioning device configured for positioning the pipetting module;
a vessel for receiving a liquid cellular sample to be analyzed;
a capacitive liquid level sensor configured for detecting the liquid level of a liquid cellular sample received within the vessel;
a reservoir comprising a pressure transmission liquid comprising an aqueous solution of at least one substance, wherein the aqueous solution has an osmolarity of 150 mOsm/l to 600 mOsm/l, and has an electrical conductivity of not more than 2 mS/cm;
a pressure transmission liquid conduit fluidically connecting the fluid sample conduit of the pipetting tip with the pressure transmission liquid reservoir; and
a pump coupled with the pressure transmission liquid conduit and configured to generate a pressure transmission liquid flow directed to the reservoir to aspirate a liquid cellular sample into the fluid sample conduit, and to generate a pressure transmission liquid flow direct to the pipetting tip to dispense liquid cellular sample from the pipetting tip.
2. The system according to claim 1 , wherein the pressure transmission liquid conduit has at least one winding inside the pipetting module.
3. A method for transferring a liquid cellular sample for analysis by a system according to claim 1 , the method comprising:
detecting a liquid level of a liquid cellular sample received within the vessel,
immersing the pipetting tip into the liquid cellular sample, and
transferring a negative pressure to the pipetting tip so as to aspirate an aliquot of the liquid cellular sample into the pipetting tip.
4. The system, according to claim 1 , wherein the aqueous solution has n osmolarity of 200 mOsm/l to 450 mOsm/l.
5. The system according to claim 4 wherein the at least one substance comprises a carbohydrate or a carbohydrate derivative.
6. The system according to claim 5 , wherein the carbohydrate or carbohydrate derivative is selected from the group consisting of a monosaccharide, a disaccharide, and an oligosaccharide.