IP Library › Granted Patent US 10,428,312
Granted Patent B2
US 10,428,312 · App. 15/101,586 · Granted Oct 1, 2019

Cytotoxic antibody

Inventors: Boon Hwa Andre Choo (Singapore, SG); Jiyun Zheng (Singapore, SG)
Assignee: Agency for Science, Technology and Research
C12N5/163C07K16/18C07K16/44C12N5/0081C07K2317/35C07K2317/565C07K2317/73
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Quick Facts
Patent No.
US 10,428,312
App. No.
15/101,586
Granted
Oct 1, 2019
Kind
B2
Abstract

An antibody that binds a glycosylated protein is disclosed, wherein the glycosylation comprises the glycan motif Fucα1-2Galβ1-3GlcNAc1-3Galβ1 or Fucα1-2Galβ1-3GlcNAc. Antibodies that are cytotoxic against undifferentiated pluripotent cells are also disclosed.

Claims (26)

1. An antibody that binds to the glycan motif Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1 or Fucα1-2Galβ1-3GlcNAc, wherein the antibody is cytotoxic against undifferentiated pluripotent cells, and wherein the antibody comprises:

at least one light chain variable region incorporating the following CDRs:

CDR1:

(SEQ ID NO: 1)

SASSSVSYMF

CDR2:

(SEQ ID NO: 2)

LTSNLAS

CDR3:

(SEQ ID NO: 3)

QQWSSNPYT;

and

at least one heavy chain variable region incorporating the following CDRs:

CDR1:

(SEQ ID NO: 4)

GFTFSNYYMN

CDR2:

(SEQ ID NO: 5)

EIRLKSNNYATHYAESVKG

CDR3:

(SEQ ID NO: 6)

FGY.

2. A monoclonal antibody produced from hybridoma cell line TAG-A1, deposited with American Type Culture Collection under Accession Number PTA-121134.

3. A hybridoma cell line TAG-A1, deposited with American Type Culture Collection under Accession Number PTA-121134.

4. The antibody of claim 1 , wherein the glycan motif is Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1.

5. The antibody of claim 1 , wherein the antibody is bivalent.

Assignments (2)
CORRECTIVE ASSIGNMENT TO CORRECT THE ADDRESS OF THE RECEIVING PARTY PREVIOUSLY RECORDED ON REEL 038799 FRAME 0399. ASSIGNOR(S) HEREBY CONFIRMS THE ASSIGNMENT. Recorded Jan 13, 2017
From: CHOO, BOON HWA ANDRE; ZHENG, JIYUN
To: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Reel/Frame 041364/0890 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 3, 2016
From: CHOO, BOON HWA ANDRE; ZHENG, JIYUN
To: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Reel/Frame 038799/0399 →
Priority Claims (1)
SG 201308964 · Dec 3, 2013 · national
Continuity (1)
Related Publication 20170002331A1 · Jan 5, 2017