Cytotoxic antibody
View Patent ↗An antibody that binds a glycosylated protein is disclosed, wherein the glycosylation comprises the glycan motif Fucα1-2Galβ1-3GlcNAc1-3Galβ1 or Fucα1-2Galβ1-3GlcNAc. Antibodies that are cytotoxic against undifferentiated pluripotent cells are also disclosed.
1. An antibody that binds to the glycan motif Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1 or Fucα1-2Galβ1-3GlcNAc, wherein the antibody is cytotoxic against undifferentiated pluripotent cells, and wherein the antibody comprises:
at least one light chain variable region incorporating the following CDRs:
CDR1:
(SEQ ID NO: 1)
SASSSVSYMF
CDR2:
(SEQ ID NO: 2)
LTSNLAS
CDR3:
(SEQ ID NO: 3)
QQWSSNPYT;
and
at least one heavy chain variable region incorporating the following CDRs:
CDR1:
(SEQ ID NO: 4)
GFTFSNYYMN
CDR2:
(SEQ ID NO: 5)
EIRLKSNNYATHYAESVKG
CDR3:
(SEQ ID NO: 6)
FGY.
2. A monoclonal antibody produced from hybridoma cell line TAG-A1, deposited with American Type Culture Collection under Accession Number PTA-121134.
3. A hybridoma cell line TAG-A1, deposited with American Type Culture Collection under Accession Number PTA-121134.
4. The antibody of claim 1 , wherein the glycan motif is Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1.
5. The antibody of claim 1 , wherein the antibody is bivalent.