GEL FOR USE IN POLYACRYLAMIDE GEL ELECTROPHORESIS AND ELECTROPHORESIS DEVICE USING SAID GEL
There are provided (i) a gel for use in polyacrylamide gel electrophoresis which gel produces a concentration effect for a longer time period and prevents heat generation during migration and (ii) an electrophoresis apparatus provided with the gel. A gel according to the present invention for use in polyacrylamide gel electrophoresis includes a concentration gel and a separation gel having a pH adjusted to a value different from the pH value of the concentration gel, an acrylamide buffer being covalently bonded to at least one of the concentration gel and the separation gel.
1 . A gel for use in polyacrylamide gel electrophoresis,
the gel comprising:
a concentration gel; and
a separation gel having a pH adjusted to a value different from a value of a pH of the concentration gel,
an acrylamide buffer being covalently bonded to at least one of the concentration gel and the separation gel.
2 . The gel for use in polyacrylamide gel electrophoresis according to claim 1 ,
wherein
the acrylamide buffer is crosslinked to the concentration gel and has a pKa within a range of 6.2 to 8.5.
3 . The gel according to claim 1 ,
wherein
the acrylamide buffer is crosslinked to the separation gel and has a pKa within a range of 8.5 to 9.3.
4 . The gel according to claim 1 ,
wherein:
the pH of the concentration gel has been adjusted to a value within a range of 6.6 to 7.5; and
the pH of the separation gel has been adjusted to a value within a range of 8.4 to 8.8.
5 . The gel according to claim 1 ,
wherein
the gel is a gel for use in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
6 . An electrophoresis apparatus, comprising:
a gel for use in polyacrylamide gel electrophoresis according to claim 1 ;
a cathode;
an anode provided opposite to the cathode with respect to the gel for use in polyacrylamide gel electrophoresis; and
a cathode buffer to be added to a portion of the gel for use in polyacrylamide gel electrophoresis which portion is on a side of the cathode.
7 . The electrophoresis apparatus according to claim 6 ,
wherein
the cathode buffer includes a front ion and a trailing ion.
8 . The electrophoresis apparatus according to claim 6 ,
wherein:
the cathode buffer has a buffer concentration adjusted to a value within a range of 20 mM to 50 mM; and
the gel has a buffer concentration adjusted to a value within a range of 150 mM to 500 mM.
9 . The electrophoresis apparatus according to claim 6 , further comprising:
a first migration section configured to carry out a first-dimension electrophoresis; and
a second migration section configured to carry out a second-dimension electrophoresis,
wherein
the second migration section includes the gel as a gel for electrophoresis.