Structured illumination microscope, structured illumination method, and program
A structured illumination microscope includes a spatial light modulator containing ferroelectric liquid crystals, an interference optical system for illuminating a specimen with an interference fringe generated by making lights from the spatial light modulator interfere with each other, a controller for applying a voltage pattern having a predetermined voltage value distribution to the ferroelectric liquid crystals, an image forming optical system for forming an image of the specimen, which has been irradiated with the interference fringe, an imaging element for generating an image by imaging the image formed by the image forming optical system, and a demodulating part for generating a demodulated image using a plurality of images, wherein the controller applies an image generation voltage pattern for generating the demodulated images and a burn-in prevention voltage pattern calculated based on the image generation voltage pattern to the ferroelectric liquid crystals.
1. A structured illumination microscope, comprising:
a spatial light modulator,
an interference optical system for illuminating a specimen with an interference fringe,
a controller for applying a voltage pattern having a predetermined voltage value distribution to the spatial light modulator,
an image forming optical system for forming an image of the specimen, which has been irradiated with the interference fringe,
an imaging element for generating an image by imaging the image formed by the image forming optical system, and
a demodulating part for generating a demodulated image using a plurality of images, wherein
the controller applies an image generation voltage pattern for generating the demodulated images and a burn-in prevention voltage pattern calculated based on a plurality of the image generation voltage patterns to the spatial light modulator.
2. The structured illumination microscope according to claim 1 , wherein
the burn-in prevention voltage pattern is not used for generating the demodulation image.
3. The structured illumination microscope according to claim 1 , wherein
the controller
applies the image generation voltage pattern in a predetermined sequence to change a phase and direction of the interference fringe, and
applies the burn-in prevention voltage pattern before and after the change in direction of the interference fringe.
4. The structured illumination microscope according to claim 1 , wherein
the burn-in prevention voltage pattern is calculated based on a reverse voltage of a sum voltage pattern that is a sum of the plurality of the image generation voltage patterns.
5. The structured illumination microscope according to claim 1 , wherein
the spatial light modulator includes a first substrate on which a plurality of pixel electrodes are provided, a second substrate facing the first substrate, and a liquid crystal arranged between the first substrate and the second substrate, and
the controller applies the voltage pattern to the spatial light modulator via the pixel electrodes.
6. The structured illumination microscope according to claim 5 , wherein
the liquid crystal is a ferroelectric liquid crystal.
7. The structured illumination microscope according to claim 1 , wherein
the image generation voltage pattern is composed of a first voltage value having a positive electric potential and a second voltage value having a negative electric potential with the same absolute value as the first voltage value.
8. The structured illumination microscope according to claim 1 , wherein
the controller
applies the image generation voltage pattern in a predetermined sequence to change a phase and a direction of the interference fringe, and
applies the burn-in prevention voltage pattern after changing the direction of the interference fringe at least twice.
9. A structured illumination method, comprising:
(a) illuminating a specimen with an interference fringe,
(b) applying a voltage pattern having a predetermined voltage value distribution to a spatial light modulator,
(c) forming an image the specimen illuminated by the interference fringe,
(d) generating an image by imaging the image formed in (c), and
(e) generating a demodulated image using a plurality of the images, wherein an image generation voltage pattern for generating the demodulated image and a burn-in prevention voltage pattern calculated based on a plurality of the image generation voltage patterns are applied to the spatial light modulator in (b).
10. A non-transitory computer readable medium storing a program for causing a computer to execute:
(a) illuminating a specimen with an interference fringe,
(b) applying a voltage pattern having a predetermined voltage value distribution to a spatial light modulator,
(c) forming an image the specimen illuminated by the interference fringe,
(d) generating an image by imaging the image formed in (c), and
(e) generating a demodulated image using a plurality of the images, wherein an image generation voltage pattern for generating the demodulated image and a burn-in prevention voltage pattern calculated based on a plurality of the image generation voltage patterns are applied to the spatial light modulator in (b).