IP Library Granted Patent US 10,100,290
Granted Patent B2
US 10,100,290 · App. 15/105,673 · Granted Oct 16, 2018

Glycosyltransferase glycosylating flavokermesic acid and/or or kermesic acid

Inventors: Rubini Maya Kannangara (Frederiksberg, DK); Mads Bennedsen (Græsted, DE); Bjørn Madsen (Helsingør, DK); Kim Binderup (Charlottelund, DK); Ulf Thrane (Helsinge, DK); Rasmus John Normand Frandsen (Allerød, DK); Uffe Hasbro Mortensen (Copenhagen N, DK); Birger Lindberg Møller (Brønshøj, DK); Finn Thyge Okkels (Roskilde, DK)
Assignees: Københavns Universitet; Danmarks Tekniske Universitet
C12N9/1048C12N1/14C12N1/20C12N9/1051C12N15/63C12N15/80C12N15/81C12N15/815C12P7/66C12Y204/00
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Quick Facts
Patent No.
US 10,100,290
App. No.
15/105,673
Granted
Oct 16, 2018
Kind
B2
Abstract

An isolated glycosyltransferase (GT) polypeptide capable of: (I): conjugating glucose to flavokermesic acid (FK); and/or (II): conjugating glucose to kermesic acid (KA) and use of this GT to e.g. make Carminic acid.

Claims (10)

1. A recombinant expression vector comprising a nucleic acid construct comprising an isolated polynucleotide comprising a nucleotide sequence which encodes an isolated glycosyltransferase polypeptide capable of:

(I): conjugating nucleotide activated glucose to flavokermesic acid (FK); and/or

(II): conjugating nucleotide activated glucose to kermesic acid (KA);

and wherein the glycosyltransferase polypeptide is at least one polypeptide selected from the group consisting of:

(a) a polypeptide comprising an amino acid sequence which has at least 95% identity with amino acids 1 to 515 of SEQ ID NO:2; and

(b) a polypeptide comprising an amino acid sequence which has at least 95% identity with amino acids 20 to 468 of SEQ ID NO:2; and

wherein said polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in an expression host.

2. A recombinant host cell comprising the recombinant expression vector of claim 1 .

3. The recombinant host cell of claim 2 , wherein the recombinant host cell is a cell selected from the group consisting of a filamentous fungal cell and a microorganism cell, and wherein if the microorganism cell is a yeast cell, the yeast cell is selected from the group consisting of Saccharomyces spp, and Pichia spp.

4. The recombinant host cell of claim 3 , wherein the recombinant host cell is Saccharomyces cerevisiae.

Assignments (7)
CHANGE OF NAME EFFECTIVE 02/01/2022 Recorded May 10, 2022
From: CHR. HANSEN NATURAL COLORS A/S
To: OTERRA A/S
Reel/Frame 059913/0725 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 19, 2016
From: CHR. HANSEN A/S
To: KOBENHAVNS UNIVERSITET; DANMARKS TEKNISKE UNIVERSITET
Reel/Frame 040060/0156 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 14, 2016
From: OKKELS, FINN THYGE
To: CHR. HANSEN A/S
Reel/Frame 040019/0284 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 7, 2016
From: CHR. HANSEN A/S
To: KOBENHAVNS UNIVERSITET; DANMARKS TEKNISKE UNIVERSITET
Reel/Frame 039101/0569 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 6, 2016
From: KANNANGARA, RUBINI MAYA; MOLLER, BIRGER LINDBERG
To: KOBENHAVNS UNIVERSITET
Reel/Frame 039080/0575 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 6, 2016
From: BENNEDSEN, MADS; MADSEN, BJORN; BINDERUP, KIM
To: CHR. HANSEN A/S
Reel/Frame 039080/0571 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 6, 2016
From: THRANE, ULF; FRANDSEN, RASMUS JOHN NORMAND; MORTENSEN, UFFE HASBRO
To: DANMARKS TEKNISKE UNIVERSITET
Reel/Frame 039081/0802 →
Priority Claims (1)
EP 13198110 · Dec 18, 2013 · regional
Continuity (1)
Related Publication 20160376569A1 · Dec 29, 2016