Flow-path control method, and cell culture device
According to the present invention, a problem of closed systems, namely minimizing the number of electromagnetic valves required to control a plurality of flow paths, can be addressed, and thus a low-cost cell culture device can be achieved. In this flow-path control method for X number of flow paths satisfying X≤2 N , the X number of flow paths are selected by using N number of valves to simultaneously and selectively control the opening and closing of the plurality of flow paths.
1. A flow-path control device comprising:
X number (X≥3) of flow paths; and
a minimum N number of valves satisfying X≤2 N ;
wherein each of the valves includes a housing, a moveable member, an actuator that is connected to drive a moveable member, a first fixed member that is fixed to the housing and has a portion extending parallel to the moveable member on a first side of the moveable member and a second fixed member that is fixed to the housing and has a portion extending parallel to the moveable member on a second side of the moveable member,
wherein, for each of the valves, the first fixed member, the second fixed member, and a portion of the moveable member that is parallel to the first fixed member and the second fixed member traverse the flow paths,
wherein each of the valves is disposed in a different position along a flow direction of the flow paths,
wherein a first plurality of the X number of flow paths pass on the first side of the moveable member between the moveable member and the first fixed member, and a second plurality of the X number of flow paths pass on the second side of the moveable member between the moveable member and the second fixed member,
wherein each of the X number of flow paths pass the respective moveable member of each valve, and
wherein the flow-path control device opens any one of the X number of flow paths by simultaneously controlling each of the N number of valves.
2. The flow-path control device according to claim 1 ,
wherein, for each of the valves, respective portions of the first fixed member, the second fixed member, and the portion of the moveable member that is parallel to the first fixed member and the second fixed member perpendicularly traverse the flow paths.
3. A cell culture device using the flow-path control device according to claim 1 , in which a supply bag for storing liquids necessary for culture and a collection bag for collecting liquid after use are connected to a culture vessel of a closed system including a lead-in port and a discharge port for fluid, the cell culture device forming a closed system and performing culture of a cell in the closed system,
wherein X number of the supply bags are present, and the supply bags are connected to one common flow path in parallel via the flow-path control device.
4. The cell culture device using the flow-path control device according to claim 3 , wherein a liquid driving source is provided in a common flow path section.
5. A cell culture device using the flow-path control device according to claim 1 , in which a supply bag for storing liquids necessary for culture and a collection bag for collecting liquid after use are connected to a culture vessel of a closed system including a lead-in port and a discharge port for fluid, the cell culture device forming a closed system and performing culture of a cell in the closed system,
wherein X number of the culture vessels are present, and the culture vessels are connected to respective common flow paths on one of the lead-in port side or the discharge port side in parallel via the flow-path control device.
6. The cell culture device using the flow-path control device according to claim 5 , wherein a liquid driving source is provided in a common flow path section.
7. A cell culture device using the flow-path control device according to claim 1 , in which a supply bag for storing liquids necessary for culture and a collection bag for collecting liquid after use are connected to a culture vessel of a closed system including a lead-in port and a discharge port for fluid, the cell culture device forming a closed system and performing culture of a cell in the closed system,
wherein X number of the collection bags are present, and the collection bags are connected to one flow path in parallel via the flow-path control device.
8. The cell culture device using the flow-path control device according to claim 7 , wherein a liquid driving source is provided in a common flow path section.
9. A cell culture device using the flow-path control device according to claim 1 , in which a supply bag for storing liquids necessary for culture and a collection bag for collecting liquid after use are connected to a culture vessel of a closed system including a lead-in port and a discharge port for fluid, the cell culture device forming a closed system and performing culture of a cell in the closed system,
wherein X number of the supply bags and X number of the culture vessels are respectively present, and the supply bags and the culture vessels are connected by the X number of flow paths.
10. A cell culture device using the flow-path control device according to claim 1 , in which a supply bag for storing liquids necessary for culture and a collection bag for collecting liquid after use are connected to a culture vessel of a closed system including a lead-in port and a discharge port for fluid, the cell culture device forming a closed system and performing culture of a cell in the closed system,
wherein X number of the culture vessels and X number of the collection bags are respectively present, and the culture vessels and the collection bags are connected by the X number of flow paths.
11. A cell culture device using the flow-path control device according to claim 2 , in which a supply bag for storing liquids necessary for culture and a collection bag for collecting liquid after use are connected to a culture vessel of a closed system including a lead-in port and a discharge port for fluid, the cell culture device forming a closed system and performing culture of a cell in the closed system,
wherein X number of the supply bags are present, and the supply bags are connected to one common flow path in parallel via the flow-path control device.
12. A cell culture device using the flow-path control device according to claim 2 , in which a supply bag for storing liquids necessary for culture and a collection bag for collecting liquid after use are connected to a culture vessel of a closed system including a lead-in port and a discharge port for fluid, the cell culture device forming a closed system and performing culture of a cell in the closed system,
wherein X number of the culture vessels are present, and the culture vessels are connected to respective common flow paths on one of the lead-in port side or the discharge port side in parallel via the flow-path control device.
13. A cell culture device using the flow-path control device according to claim 2 , in which a supply bag for storing liquids necessary for culture and a collection bag for collecting liquid after use are connected to a culture vessel of a closed system including a lead-in port and a discharge port for fluid, the cell culture device forming a closed system and performing culture of a cell in the closed system,
wherein X number of the collection bags are present, and the collection bags are connected to one flow path in parallel via the flow-path control device.
14. A cell culture device using the flow-path control device according to claim 2 , in which a supply bag for storing liquids necessary for culture and a collection bag for collecting liquid after use are connected to a culture vessel of a closed system including a lead-in port and a discharge port for fluid, the cell culture device forming a closed system and performing culture of a cell in the closed system,
wherein X number of the supply bags and X number of the culture vessels are respectively present, and the supply bags and the culture vessels are connected by the X number of flow paths.
15. A cell culture device using the flow-path control device according to claim 2 , in which a supply bag for storing liquids necessary for culture and a collection bag for collecting liquid after use are connected to a culture vessel of a closed system including a lead-in port and a discharge port for fluid, the cell culture device forming a closed system and performing culture of a cell in the closed system,
wherein X number of the culture vessels and X number of the collection bags are respectively present, and the culture vessels and the collection bags are connected by the X number of flow paths.