Therapeutic agents and delivery with microspheres
The invention relates to methods for attaching therapeutic agents to structures produced by thermally induced phase separation as well as methods for coating devices and producing multi-layered microspheres.
1. A method for attaching a therapeutic agent to a hydrophobic microsphere structure produced by thermally induced phase separation comprising:
i) at least partly submerging the hydrophobic microsphere structure in a culture medium;
ii) subsequently contacting the hydrophobic microsphere structure with a wetting agent that wets the hydrophobic microsphere structure, wherein the wetting agent is ethanol which is added to the culture medium to a concentration of from 10% to 30%; and
iii) attaching the therapeutic agent to the hydrophobic microsphere structure.
2. The method of claim 1 which results in the hydrophobic microsphere structure being fully submerged in the culture medium.
3. The method of claim 1 wherein the hydrophobic microsphere structure comprises poly(lactide-co-glycolide) (PLGA).
4. The method of claim 1 wherein the culture medium is Dulbecco's Modified Eagle's Medium (DMEM).
5. The method of claim 1 wherein the culture medium is tissue culture medium comprising serum.
6. The method of claim 1 wherein the hydrophobic microsphere structure is mixed with the culture medium before and after the contacting with the wetting agent.
7. The method of claim 1 further comprising incubating the hydrophobic microsphere structure at about 30° C. to about 40° C.
8. The method of claim 1 wherein the therapeutic agent is a cell.
9. The method of claim 1 wherein the therapeutic agent is an active pharmaceutical ingredient (API).
10. The method of claim 1 further comprising periods of static incubation interspersed with agitation.
11. The method of claim 10 comprising about 1 minute of agitation at about 300 rpm per hour of static incubation.
12. The method of claim 5 wherein the serum is fetal bovine serum (FBS) or fetal calf serum (FCS).