Method of producing sugar liquid
A method of producing a sugar liquid from cellulose-containing biomass includes step (1) of hydrolyzing cellulose-containing biomass by a filamentous fungus-derived cellulase; and step (2) of filtering a hydrolysate obtained in step (1) through an ultrafiltration membrane to recover the filamentous fungus-derived cellulase as a non-permeate and to obtain a sugar liquid as a permeate, wherein the cellulose-containing biomass is treated with one or more enzymes selected from the group consisting of pectinase, glucoamylase, and lipase at a stage previous to step (1) or in step (1).
1. A method of producing a sugar liquid from cellulose-containing biomass, comprising:
step (1) of hydrolyzing the cellulose-containing biomass with a cellulase selected from the group consisting of a cellulase derived from a filamentous fungus, a cellulase derived from the genus Cellulomonas , a cellulase derived from the genus Chlostridium and a cellulase derived from the genus Streptomyces;
step (2) of filtering a hydrolysate obtained in step (1) through an ultrafiltration membrane to recover the cellulase as a non-permeate and obtain a sugar liquid as a permeate; and
a step (3) of recycling enzymes recovered in the non-permeate in step (2) for use in step (1);
wherein the cellulose-containing biomass is treated with lipase, pectinase, and glucoamylase in step (1).
2. The method according to claim 1 , wherein said cellulose-containing biomass is one or more biomasses selected from the group consisting of grain hull biomass, straw, and bagasse.
3. The method according to claim 2 , wherein said grain hull biomass is one or more biomasses selected from the group consisting of corn hull, soybean hull, and wheat hull.
4. The method according to claim 1 , wherein the weight of one or more enzymes selected from the group consisting of said lipase, pectinase, and glucoamylase is 1/10 or less based on the weight of the cellulase.
5. A method of producing a sugar liquid from cellulose-containing biomass, comprising:
step (1) of hydrolyzing the cellulose-containing biomass with a cellulase selected from the group consisting of a cellulase derived from a filamentous fungus, a cellulase derived from the genus Cellulomonas , a cellulase derived from the genus Chlostridium and a cellulase derived from the genus Streptomyces , and increasing in an amount of recoverable cellulase in a hydrolysate by treating the cellulose-containing biomass with lipase, pectinase, and glucoamylase; and
step (2) of filtering the hydrolysate obtained in step (1) through an ultrafiltration membrane to recover the cellulase as a non-permeate and obtain a sugar liquid as a permeate.