Plants with enhanced photosynthesis and methods of manufacture thereof
A transgenic plant having enhanced photosynthesis is disclosed. The transgenic plant is transformed with a transgenic polynucleotide encoding a heterologous bicarbonate transporter. The bicarbonate transporter can be from an algae or a cyanobacterial species. The transgenic polynucleotide comprises a nucleic acid sequence encoding the bicarbonate transporter under the control of a functional plant promoter and optionally includes a chloroplast envelope targeting peptide heterologous to the bicarbonate transporter. Methods of making the transgenic plant and transgenic polynucleotide are disclosed.
1. A transgenic plant transformed with a recombinant DNA construct comprising a plant-expressible transcription regulatory sequence operatively linked to a polynucleotide encoding an algal CCP1 or CCP2 polypeptide comprising an amino acid sequence that is at least 80% homologous to SEQ ID NO: 6, wherein the transgenic plant has a CO 2 assimilation rate at least 5% higher than a plant of the same species not transformed with the recombinant DNA construct and
a reduced transpiration rate at least 5% lower than a plant of the same species not transformed with the recombinant DNA construct.
2. The transgenic plant of claim 1 , wherein the algae is a Chlamydomonas species.
3. The transgenic plant of claim 1 , wherein the CCP1 polypeptide comprises the amino acid sequence of SEQ ID NO:6.
4. The transgenic plant of claim 1 , which is an oil crop plant selected from the group consisting of Borago officinalis, Brassica campestris, Brassica napus, Brassica rapa, Camelina species, Cannabis sativa, Carthamus tinctorius, Cocos nucifera, Crambe abyssinica, Cuphea species, Elaeis guinensis, Elaeis oleifera, Glycine max, Gossypium hirsutum, Gossypium barbadense, Gossypium herbaceum, Helianthus annuus, Linum usitatissimum, Oenothera biennis, Olea europaea, Oryza sativa, Ricinus communis, Sesamum indicum, Triticum species, Zea mays , walnut and almond.
5. The transgenic plant of claim 4 , wherein the plant is Camelina sativa.
6. A method of producing a transformed plant having enhanced photosynthesis, the method comprising
transforming a plant cell with a recombinant polynucleotide comprising a nucleic acid sequence encoding an algal CCP1 or CCP2 polypeptide, comprising an amino acid sequence that is at least 80% homologous to SEQ ID NO:6, operatively linked to a plant-expressible promoter, wherein the nucleic acid sequence encoding the algal CCP1 or CCP2 polypeptide is further operatively linked to a transcription terminator;
growing a plant from the plant cell until the plant produces seed; and
selecting seeds from a plant in which the transgenic plant has a CO 2 assimilation rate higher than a plant of the same species not comprising the algal CCP1 or CCP2 polypeptide and a reduced transpiration rate lower than a plant of the same species not comprising the algal CCP1 or CCP2 polypeptide.
7. The method of claim 6 , wherein the plant is an oil crop plant selected from the group consisting of Borago officinalis, Brassica campestris, Brassica napus, Brassica rapa, Camelina species, Cannabis sativa, Carthamus tinctorius, Cocos nucifera, Crambe abyssinica, Cuphea species, Elaeis guinensis, Elaeis oleifera, Glycine max, Gossypium hirsutum, Gossypium barbadense, Gossypium herbaceum, Helianthus annuus, Linum usitatissimum, Oenothera biennis, Olea europaea, Oryza sativa, Ricinus communis, Sesamum indicum, Triticum species, Zea mays , walnut and almond.
8. The method of claim 7 , wherein the plant is a Camelina.
9. The method of claim 6 , wherein the transgenic plant has a CO 2 assimilation rate at least 5% higher than a plant of the same species not comprising the algal CCP1 or CCP2 polypeptide and a reduced transpiration rate at least 5% lower than a plant of the same species not comprising the algal CCP1 or CCP2 polypeptide.
10. The method of claim 6 wherein the CCP1 polypeptide comprises the amino acid sequence of SEQ ID NO:6.