IP Library › Granted Patent US 10,287,563
Granted Patent B2
US 10,287,563 · App. 15/109,696 · Granted May 14, 2019

Process for degrading mannan-containing cellulosic materials

Inventors: Harry Showmaker (Raleigh, NC); Laerke Tvedebrink Haahr (Bagsvaerd, DK); Kristian Bertel Romer M. Krogh (Bagsvaerd, DK); Johan Belfrage (Cupar, GB); Lone Baekgaard (Frederiksberg, DK); Armindo Ribiero Gaspar (Rolesville, NC); Claudia Geddes (Raleigh, NC)
Assignee: Novozymes A/S
C12N9/2488C12N9/2434C12N9/2445C12P7/00C12P19/02C12P19/14C12P2203/00
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Quick Facts
Patent No.
US 10,287,563
App. No.
15/109,696
Granted
May 14, 2019
Kind
B2
Abstract

The present invention relates to processes comprising enzymatic degradation of mannan-containing cellulosic materials for producing a hydrolyzate. The invention also relates to processes of producing a fermentation product from mannan-containing cellulosic materials.

Claims (17)

1. A process for degrading a mannan-containing cellulosic material, comprising: treating the cellulosic material with an enzyme composition comprising one or more cellulases, a beta-mannosidase, and at least one mannanase, wherein the mannanase has 100% sequence identity to SEQ ID NO:34.

2. The process of claim 1 , wherein the enzyme composition further comprises one or more of an AA9 polypeptide, a beta-xylosidase, a cellobiohydrolase I and a cellobiohydrolase II, a xylanase, or a beta-mannosidase.

3. The process of claim 1 , further comprising pretreating the mannan-containing cellulosic material prior to treatment of the mannan-containing cellulosic material.

4. The process of claim 1 , wherein the mannan-containing cellulosic material is municipal solid waste.

5. The process of claim 1 , further comprising recovering the degraded cellulosic material.

6. The process of claim 5 , wherein the degraded cellulosic material is a sugar.

7. A process for producing a fermentation product, comprising:

(a) saccharifying a mannan-containing cellulosic material with an enzyme composition comprising one or more cellulases, a beta-mannosidase, and at least one mannanase, wherein the mannanase has 100% sequence identity to SEQ ID NO:34;

(b) fermenting the saccharified cellulosic material with a fermenting microorganism to produce the fermentation product; and

(c) recovering the fermentation product from the fermentation.

8. The process of claim 7 , wherein the enzyme composition further comprises one or more of an AA9 polypeptide, a beta-xylosidase, a cellobiohydrolase I and a cellobiohydrolase II, a xylanase, or a beta-mannosidase.

9. The process of claim 7 , further comprising pretreating the mannan-containing cellulosic material prior to saccharification.

10. The process of claim 7 , wherein the fermentation product is an alcohol, an alkane, a cycloalkane, an alkene, an amino acid, a gas, isoprene, a ketone, an organic acid, or polyketide.

11. A process for producing a fermentation product, the process comprising

(a) contacting an aqueous slurry of a mannan-containing cellulosic material with an enzyme composition to produce a soluble hydrolyzate, wherein the enzyme composition comprises one or more cellulases, a beta-mannosidase, and at least one mannanase, wherein the mannanase has 100% sequence identity to SEQ ID NO:34; and

(b) contacting the soluble hydrolyzate with a fermenting organism to produce a fermentation product.

12. The process of claim 11 , wherein the enzyme composition further comprises one or more of an AA9 polypeptide, a beta-xylosidase, a cellobiohydrolase I and a cellobiohydrolase II, a xylanase, or a beta-mannosidase.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 22, 2016
From: HAAHR, LAERKE TVEDBRINK
To: NOVOZYMES A/S
Reel/Frame 039827/0842 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 5, 2016
From: SHOWMAKER, HARRY; HAAHR, TVEDBRINK LAERKE; KROGH, KRISTIAN BERTEL, ROMER M.; BELFRAGE, JOHAN; BAEKGAARD, LONE; GASPAR, ARMINDO RIBIERO; GEDDES, CLAUDIA
To: NOVOZYMES A/S
Reel/Frame 039073/0451 →
Continuity (2)
Provisional Application 61924491 · Jan 7, 2014
Related Publication 20160333333A1 · Nov 17, 2016