IP Library Granted Patent US 10,487,308
Granted Patent B2
US 10,487,308 · App. 15/113,762 · Granted Nov 26, 2019

Culture medium composition

Inventors: Hisato Hayashi (Funabashi, JP); Misayo Otani (Funabashi, JP); Koichiro Saruhashi (Funabashi, JP); Taito Nishino (Shiraoka, JP); Takehisa Iwama (Funabashi, JP); Tatsuro Kanaki (Shiraoka, JP); Ayako Aihara (Shiraoka, JP)
Assignee: NISSAN CHEMICAL CORPORATION
C12N5/0068C12N2533/70C12N2533/72C12N2533/78C12N2535/00
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Quick Facts
Patent No.
US 10,487,308
App. No.
15/113,762
Granted
Nov 26, 2019
Kind
B2
Abstract

The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues without substantially increasing the viscosity of the solution by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.

Claims (1)

1. A method of proliferating an adherent cell, comprising suspension culturing the adherent cell in a medium composition comprising a chitin nanofiber attached to the adherent cell, wherein the medium composition has a chitin nanofiber content of 0.001% (weight/volume) to 0.1% (weight/volume).

Assignments (3)
CHANGE OF NAME AND CHANGE OF ADDRESS Recorded Oct 15, 2019
From: NISSAN CHEMICAL INDUSTRIES, LTD.
To: NISSAN CHEMICAL CORPORATION
Reel/Frame 050727/0512 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 21, 2016
From: KYOTO UNIVERSITY
To: NISSAN CHEMICAL INDUSTRIES, LTD.
Reel/Frame 040394/0092 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 23, 2016
From: HAYASHI, HISATO; OTANI, MISAYO; SARUHASHI, KOICHIRO; NISHINO, TAITO; IWAMA, TAKEHISA; KANAKI, TATSURO; AIHARA, AYAKO
To: NISSAN CHEMICAL INDUSTRIES, LTD.; KYOTO UNIVERSITY
Reel/Frame 039847/0633 →
Priority Claims (4)
JP 2014-010842 · Jan 23, 2014 · national
JP 2014-123772 · Jun 16, 2014 · national
JP 2014-174574 · Aug 28, 2014 · national
JP 2014-217761 · Oct 24, 2014 · national
Continuity (1)
Related Publication 20170009201A1 · Jan 12, 2017