Chimeric antigen receptors and methods of making
Provided are methods of generating chimeric antigen receptors (CAR). In some embodiments, library screening of CAR is performed by generating a vector encoding the CAR from random attachment of vectors from libraries of vectors encoding antigen-binding domains (e.g., scFv regions), hinge regions, and endodomains. In some embodiments, the vectors contain a transposon.
1. A nucleic acid encoding a chimeric antigen receptor comprising CAR 213 (SEQ ID NO:5).
2. The nucleic acid of claim 1 , wherein the nucleic acid is comprised in a T cell.
3. The nucleic acid of claim 2 , wherein the T cell is an alpha beta T cell, a gamma delta T cell, or a NKT cell.
4. A Sleeping Beauty transposon comprising a nucleic acid encoding a chimeric antigen receptor comprising CAR 213 (SEQ ID NO: 5).
5. A transformed T cell comprising a nucleic acid encoding a chimeric antigen receptor encoded by SEQ ID NO: 5.
6. The transformed T cell of claim 5 further comprising a membrane-bound cytokine.
7. The transformed T cell of claim 6 , wherein the membrane-bound cytokine is membrane-bound IL-15.