Ultrafiltration unit for continuous buffer or medium exchange from a protein solution
The invention relates to an ultrafiltration unit for continuous buffer or medium exchange, a method for continuous buffer or medium exchange in the ultrafiltration unit, and a plant in particular for (semi)continuous production of biopharmaceutical and biological macromolecular products in particular, such as proteins, e.g. monoclonal antibodies and vaccines, comprising the ultrafiltration unit according to the invention.
1. A method for continuous ultrafiltration of a feed stream containing biopharmaceutical and biological macromolecular products in a unit for continuous ultrafiltration, wherein the feed stream is washed with a washing fluid via at least one capillary ultrafiltration membrane of a capillary ultrafiltration module, wherein the feed stream is conveyed into the capillary and the washing fluid is conveyed over the outside of the capillary, wherein the feed stream and the washing fluid are continuously fed into the ultrafiltration module, and are continuously removed from the ultrafiltration module, wherein the feed stream and the washing fluid is not circulated into the capillary ultrafiltration module and the removal of a product stream comprising a retentate is regulated such that no undesired net flows can pass from the capillary interior to the capillary exterior or vice versa,
wherein the capillary ultrafiltration module is bubble free on both a feed side and a jacket side via a priming procedure in which both the feed side and the jacket side are flushed with buffer solution until no more gas bubbles escape from the ultrafiltration module,
and wherein both the feed side and the jacket side are flushed by the buffer solution at a flow rate ranging between 10% and 100% of a blood flow rate.
2. The method according to claim 1 , wherein in the unit for continuous ultrafiltration, product in the feed stream is at most concentrated by a factor of 2.
3. The method according to claim 1 , wherein the washing fluid is passed in cross-current configuration to the feed stream.
4. The method according to claim 1 , wherein a plurality of the at least one capillary ultrafiltration modules is connected in series and the maximum pressure drop over membranes of the capillary ultrafiltration modules does not exceed 1 bar.
5. The method according to claim 1 , wherein in the unit for continuous ultrafiltration, product in the feed stream is at most diluted by a factor of 2.
6. The method according to claim 1 , wherein the washing fluid is passed in countercurrent configuration to the feed stream.
7. The method according to claim 1 , wherein a plurality of the at least one capillary ultrafiltration modules are connected in parallel and the maximum pressure drop over membranes of the capillary ultrafiltration modules does not exceed 1 bar.
8. The method according to claim 1 wherein the flow rate ranges between 15% and 100% of the blood flow rate.
9. The method according to claim 1 wherein the flow rate ranges between 30% and 100% of the blood flow rate.